2--deoxytubercidin and 5-chloro-2--deoxyuridine

2--deoxytubercidin has been researched along with 5-chloro-2--deoxyuridine* in 2 studies

Other Studies

2 other study(ies) available for 2--deoxytubercidin and 5-chloro-2--deoxyuridine

Article	Year
Chemical Morphing of DNA Containing Four Noncanonical Bases. Angewandte Chemie (International ed. in English), 2016, 06-20, Volume: 55, Issue:26 The ability of alternative nucleic acids, in which all four nucleobases are substituted, to replicate in vitro and to serve as genetic templates in vivo was evaluated. A nucleotide triphosphate set of 5-chloro-2'-deoxyuridine, 7-deaza-2'-deoxyadenosine, 5-fluoro-2'-deoxycytidine, and 7-deaza-2'deoxyguanosine successfully underwent polymerase chain reaction (PCR) amplification using templates of different lengths (57 or 525mer) and Taq or Vent (exo-) DNA polymerases as catalysts. Furthermore, a fully morphed gene encoding a dihydrofolate reductase was generated by PCR using these fully substituted nucleotides and was shown to transform and confer trimethoprim resistance to E. coli. These results demonstrated that fully modified templates were accurately read by the bacterial replication machinery and provide the first example of a long fully modified DNA molecule being functional in vivo. Topics: Deoxycytidine; Deoxyguanine Nucleotides; Deoxyuridine; DNA; Escherichia coli; Polymerase Chain Reaction; Trimethoprim; Trimethoprim Resistance; Tubercidin	2016
The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair. Organic & biomolecular chemistry, 2016, Dec-20, Volume: 15, Issue:1 5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2'-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2'-deoxyuridine and 7-deaza-2'-deoxyadenosine combination and with Taq DNA polymerase. Topics: Adenine; Base Pairing; Deoxyadenosines; Deoxyuridine; DNA; Tubercidin; Uracil	2016

Article

Year

Chemical Morphing of DNA Containing Four Noncanonical Bases.

Angewandte Chemie (International ed. in English), 2016, 06-20, Volume: 55, Issue:26

The ability of alternative nucleic acids, in which all four nucleobases are substituted, to replicate in vitro and to serve as genetic templates in vivo was evaluated. A nucleotide triphosphate set of 5-chloro-2'-deoxyuridine, 7-deaza-2'-deoxyadenosine, 5-fluoro-2'-deoxycytidine, and 7-deaza-2'deoxyguanosine successfully underwent polymerase chain reaction (PCR) amplification using templates of different lengths (57 or 525mer) and Taq or Vent (exo-) DNA polymerases as catalysts. Furthermore, a fully morphed gene encoding a dihydrofolate reductase was generated by PCR using these fully substituted nucleotides and was shown to transform and confer trimethoprim resistance to E. coli. These results demonstrated that fully modified templates were accurately read by the bacterial replication machinery and provide the first example of a long fully modified DNA molecule being functional in vivo.

Topics: Deoxycytidine; Deoxyguanine Nucleotides; Deoxyuridine; DNA; Escherichia coli; Polymerase Chain Reaction; Trimethoprim; Trimethoprim Resistance; Tubercidin

2016

The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair.

Organic & biomolecular chemistry, 2016, Dec-20, Volume: 15, Issue:1

5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2'-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2'-deoxyuridine and 7-deaza-2'-deoxyadenosine combination and with Taq DNA polymerase.

Topics: Adenine; Base Pairing; Deoxyadenosines; Deoxyuridine; DNA; Tubercidin; Uracil

2016