2--acetylacteoside and acteoside

Phytochemical investigation on the EtOH extract from the aerial part of Callicarpa kwangtungensis led to the isolation and characterization of 10 caffeoyl phenylethanoid glycosides, 2'-acetylacteoside (1), tubuloside E (2), acteoside (3), tubuloside B (4), isoacteoside (5), alyssonoside (6), 2'-acetylforsythoside B (7), brandioside (8), forsythoside B (9), and poliumoside (10). Compound 4 was isolated from the plants of Verbenaceae,and 6 was obtained from the Callicarpa genus, for the first time, while compounds 1, 2, 5 and 7 were firstly reported from the plant.

Topics: Caffeic Acids; Catechols; Chromatography, High Pressure Liquid; Ethanol; Glucosides; Glycosides; Phenols; Verbenaceae

To have a contrast study on phenylethanoid glycosides from Cistanche deserticola Y. C. Ma collected in different seasons.. LC/MS method has been applied for the analysis of four kinds of phenylethanoid glycosides compunds (echinacoside, acteoside, cistanoside A and 2'-acetylacteoside) from Cistanche deserticola Y. C. Ma in spring and autumn.. According to the special MS spectra and HPLC chromatogram, this four kinds of phenylethanoid glycosides compounds were detected in each Cistanche deserticola Y. C. Ma, but the content is considerable different except the acteoside.. The content of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma in different seasons has a difference from each other, the quality of Cistanche deserticola Y. C. Ma is also different.

Topics: Chromatography, Liquid; Cistanche; Gas Chromatography-Mass Spectrometry; Glucosides; Glycosides; Phenols; Plant Stems; Plants, Medicinal; Seasons

High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of phenylethanoid glycosides (PhGs) acteoside and 2'-acetylacteoside from Cistanches salsa (C.A. Mey) G. Beck with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (4:0.6:0.6:5, v/v). HPLC analyses of the CCC fractions revealed that the two main PhGs were over 98% purity. Their chemical structures were identified by 1H NMR, 13C NMR and MS.

Topics: Chromatography, High Pressure Liquid; Countercurrent Distribution; Glucosides; Magnetic Resonance Spectroscopy; Magnoliopsida; Phenols

Four phenylethanoids isolated from the stems of Cistanche deserticola, acteoside (1), 2'-acetylacteoside (2), isoacteoside (3) and tubuloside B (4), significantly suppressed NADPH/CCl4-induced lipid peroxidation in rat liver microsomes. Addition of them to primary cultured rat hepatocytes efficiently prevented cell damage induced by exposure to CCl4 or D-galactosamine (D-GalN). Acteoside (1) further showed pronounced anti-hepatotoxic activity against CCl4 in vivo.

Topics: Animals; Carbon Tetrachloride; Cell Survival; Cells, Cultured; Glucosides; Lipid Peroxidation; Liver; Male; Medicine, Chinese Traditional; Microsomes, Liver; Molecular Structure; Phenols; Plant Extracts; Plants, Medicinal; Rats; Rats, Wistar