2--3--o-(2-4-6-trinitrophenyl)adenosine-5--triphosphate and beta--gamma-methylenedeoxythymidine-5--triphosphate

The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion (DRG) neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist beta, gamma-me-ATP and protein kinase C (PKC) on P2X receptor-mediated inward current in DRG neurons.. Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats.. beta, gamma-me-ATP, similar as ATP, evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12, 13-dibutyrate (PDBu) significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner.. Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.

Topics: Adenosine Triphosphate; Analysis of Variance; Animals; Dose-Response Relationship, Drug; Drug Interactions; Electric Stimulation; Enzyme Activation; Enzyme Inhibitors; Ganglia, Spinal; Indoles; Male; Maleimides; Membrane Potentials; Neurons; Patch-Clamp Techniques; Phorbol 12,13-Dibutyrate; Protein Kinase C; Purinergic P2 Receptor Agonists; Purinergic P2 Receptor Antagonists; Rats; Rats, Sprague-Dawley; Receptors, Purinergic P2; Receptors, Purinergic P2X2; Thymine Nucleotides