16-16-dimethylprostaglandin-e2 and sofalcone

The effects of 16,16-dimethylprostaglandin E2 (dm-PGE2) and sofalcone on damage caused by ethanol in surface epithelial cells isolated from rat stomach were examined. The surface epithelial cells (SECs) accounted for 83% of the isolated gastric mucosal cells, mucus neck cells for 5%, parietal cells for 9%, and chief cells for 3%. To suspensions of SEC, either dm-PGE2 at concentrations of 10(-7), 10(-6), or 10(-5) M or sofalcone at concentrations from 5 X 10(-6) to 10(-4) M was added; some cells were treated with neither drug. Ten minutes later, ethanol was added to each cell suspension to a final concentration of 15%, and 5 min later the viability was evaluated by trypan blue exclusion. At 10(-6) M, dm-PGE2 reduced ethanol-induced cell damage most strongly (p less than 0.001). Sofalcone also helped to prevent cell damage caused by ethanol in a dose-dependent manner. These results suggest that dm-PGE2 and sofalcone protect gastric mucosa not only from gross visible damage but they directly protect gastric cells from damage.

Topics: 16,16-Dimethylprostaglandin E2; Animals; Anti-Ulcer Agents; Cells, Cultured; Chalcone; Chalcones; Cimetidine; Ethanol; Gastric Mucosa; In Vitro Techniques; Male; Microscopy, Electron, Scanning; Rats; Rats, Inbred Strains

We studied the effects of 16,16-dimethyl prostaglandin E2 (dm-PGE2) and sofalcone, a new antiulcer agent developed in Japan, on ethanol damage to isolated surface epithelial cells (SEC) in vitro and gastric mucosa of rats in vivo. Rats were given 5 micrograms/kg dm-PGE2, 30, 100, or 300 mg/kg sofalcone, or the vehicle, intraperitoneally. In the in vitro study, damage of the SEC isolated from rats given dm-PGE2 or sofalcone was significantly less after exposure to 15% ethanol than for the SEC from the control rats. In the in vivo study, the 15% ethanol did not induce gross visible damage, but did cause surface epithelial damage in the control rats as judged by scanning electron microscopy. This damage was inhibited by dm-PGE2 or sofalcone. Damage from absolute ethanol was inhibited by both of the agents as judged by the gross appearance, but the surface epithelium was damaged in all rats. We concluded that dm-PGE2 and sofalcone protect gastric mucosa from gross damage caused by absolute ethanol, and protect SEC both in vivo and in vitro from being damaged by ethanol when the concentration of ethanol is 15%.

Topics: 16,16-Dimethylprostaglandin E2; Animals; Anti-Ulcer Agents; Chalcone; Chalcones; Ethanol; Gastric Mucosa; Indomethacin; Male; Propiophenones; Prostaglandins E, Synthetic; Rats; Rats, Inbred Strains