15-ketoprostaglandin-e2 and 13-hydroperoxy-9-11-octadecadienoic-acid

15-ketoprostaglandin-e2 has been researched along with 13-hydroperoxy-9-11-octadecadienoic-acid* in 1 studies

Other Studies

1 other study(ies) available for 15-ketoprostaglandin-e2 and 13-hydroperoxy-9-11-octadecadienoic-acid

Article	Year
Inhibition of 15-hydroxy prostaglandin dehydrogenase activity in rabbit gastric antral mucosa by 13-hydroperoxyoctadecadienoic acid. Prostaglandins, leukotrienes, and essential fatty acids, 1994, Volume: 51, Issue:6 The effect of 13-hydroperoxyoctadecadienoic acid (13-HPODE), a hydroperoxy adduct of linoleic acid (LA), on the activities of prostaglandin (PG) synthesizing and catabolizing enzymes in rabbit gastric antral mucosa was examined. 13-HPODE had no effect on the synthesis of PGE2, PGF2 alpha and PGD2 from exogenous arachidonic acid in the microsomal fraction of the gastric mucosa at concentrations ranging from 5-20 microM. On the other hand, at 1-10 microM, it inhibited the activity of 15-hydroxy PG dehydrogenase (PGDH), which catalyzes the initial step of catabolism of PGs, in a dose-dependent manner. The concentration required for 50% inhibition was approximately 1 microM. Experiments utilizing LA, 13-hydroperoxyoctadecadienoic acid and Fe2+ indicated the requirement of the hydroperoxy moiety for the inhibitory effect of 13-HPODE on the PGDH activity. These results suggest that 13-HPODE has the potential to increase the levels of biologically active PGs in gastric mucosa by preventing their inactivation and may have functional effects within the stomach. Topics: Animals; Arachidonic Acid; Chromatography, High Pressure Liquid; Cytosol; Dinoprost; Dinoprostone; Gastric Mucosa; Hydroxyprostaglandin Dehydrogenases; Linoleic Acids; Lipid Peroxides; Male; Microsomes; Prostaglandin D2; Prostaglandin-Endoperoxide Synthases; Prostaglandins; Pyloric Antrum; Rabbits	1994

Article

Year

Inhibition of 15-hydroxy prostaglandin dehydrogenase activity in rabbit gastric antral mucosa by 13-hydroperoxyoctadecadienoic acid.

Prostaglandins, leukotrienes, and essential fatty acids, 1994, Volume: 51, Issue:6

The effect of 13-hydroperoxyoctadecadienoic acid (13-HPODE), a hydroperoxy adduct of linoleic acid (LA), on the activities of prostaglandin (PG) synthesizing and catabolizing enzymes in rabbit gastric antral mucosa was examined. 13-HPODE had no effect on the synthesis of PGE2, PGF2 alpha and PGD2 from exogenous arachidonic acid in the microsomal fraction of the gastric mucosa at concentrations ranging from 5-20 microM. On the other hand, at 1-10 microM, it inhibited the activity of 15-hydroxy PG dehydrogenase (PGDH), which catalyzes the initial step of catabolism of PGs, in a dose-dependent manner. The concentration required for 50% inhibition was approximately 1 microM. Experiments utilizing LA, 13-hydroperoxyoctadecadienoic acid and Fe2+ indicated the requirement of the hydroperoxy moiety for the inhibitory effect of 13-HPODE on the PGDH activity. These results suggest that 13-HPODE has the potential to increase the levels of biologically active PGs in gastric mucosa by preventing their inactivation and may have functional effects within the stomach.

Topics: Animals; Arachidonic Acid; Chromatography, High Pressure Liquid; Cytosol; Dinoprost; Dinoprostone; Gastric Mucosa; Hydroxyprostaglandin Dehydrogenases; Linoleic Acids; Lipid Peroxides; Male; Microsomes; Prostaglandin D2; Prostaglandin-Endoperoxide Synthases; Prostaglandins; Pyloric Antrum; Rabbits

1994