15-hydroxy-5-8-11-13-eicosatetraenoic-acid and triethylamine

15-hydroxy-5-8-11-13-eicosatetraenoic-acid has been researched along with triethylamine* in 1 studies

Other Studies

1 other study(ies) available for 15-hydroxy-5-8-11-13-eicosatetraenoic-acid and triethylamine

ArticleYear
Solid-phase extraction and high-performance liquid chromatography analysis of lipoxygenase pathway products.
    Analytical biochemistry, 1986, Volume: 154, Issue:1

    Liquid chromatography methods for quantitation of leukotrienes and HETEs (hydroxyeicosatetraenoic acids) in biological samples are described. Extraction is accomplished by acetonitrile precipitation of proteins followed by selective acetonitrile elution from a solid-phase C18 extraction cartridge. Isocratic elution of extracts from short reverse-phase columns with 3 microns C18-bonded silica results in elution of all components of interest in less than 10 min. The addition of the mobile-phase additives, trifluoroacetic acid and triethylamine, enable the peptido-leukotrienes to be eluted with excellent peak shape and unique elution times. As little as 1 pmol of each metabolite can be detected by uv spectrophotometry with a wavelength change from 280 to 235 nm midway through the chromatographic run. This method is demonstrated by the extraction and analysis of leukotriene and HETE standards from human serum.

    Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Arachidonate Lipoxygenases; Chromatography, High Pressure Liquid; Ethylamines; Humans; Hydroxyeicosatetraenoic Acids; Leukotriene B4; Lipoxygenase; SRS-A; Trifluoroacetic Acid

1986