12-oxophytodienoic-acid and indoleacetic-acid

A highly sensitive and accurate multiplex gas chromatography-mass spectrometry (GC-MS/MS)-technique is reported for the quantitative analysis of acidic phytohormones in Arabidopsis thaliana and other plant species. The optimized setup allows the routine processing and analysis of between 20 mg and 5 g of tissue. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signaling molecules.

Topics: Abscisic Acid; Arabidopsis; Cyclopentanes; Diazomethane; Fatty Acids, Unsaturated; Gas Chromatography-Mass Spectrometry; Indoleacetic Acids; Oxylipins; Plant Growth Regulators; Plant Proteins; Salicylic Acid

A highly sensitive and accurate multiplex gas chromatography-tandem mass spectrometry (GC-MS/MS) technique is reported for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid. The optimized setup allows the routine processing and analysis of up to 60 plant samples of between 20 and 200 mg of fresh weight per day. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signalling molecules. Whole-plant organ-distribution maps for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid were generated for Arabidopsis thaliana (L.) Heynh. For leaves of A. thaliana, a spatial resolution of hormone quantitation down to approximately 2 mm(2) was achieved.

Topics: Abscisic Acid; Algorithms; Arabidopsis; Biological Transport; Cyclopentanes; Fatty Acids, Unsaturated; Gas Chromatography-Mass Spectrometry; Indoleacetic Acids; Molecular Structure; Oxylipins; Plant Growth Regulators; Salicylic Acid; Signal Transduction