12-hydroxy-5-8-10-14-eicosatetraenoic-acid and xanthohumol

12-hydroxy-5-8-10-14-eicosatetraenoic-acid has been researched along with xanthohumol* in 1 studies

Other Studies

1 other study(ies) available for 12-hydroxy-5-8-10-14-eicosatetraenoic-acid and xanthohumol

ArticleYear
Xanthohumol attenuates tumour cell-mediated breaching of the lymphendothelial barrier and prevents intravasation and metastasis.
    Archives of toxicology, 2013, Volume: 87, Issue:7

    Health beneficial effects of xanthohumol have been reported, and basic research provided evidence for anti-cancer effects. Furthermore, xanthohumol was shown to inhibit the migration of endothelial cells. Therefore, this study investigated the anti-metastatic potential of xanthohumol. MCF-7 breast cancer spheroids which are placed on lymphendothelial cells (LECs) induce "circular chemorepellent-induced defects" (CCIDs) in the LEC monolayer resembling gates for intravasating tumour bulks at an early step of lymph node colonisation. NF-κB reporter-, EROD-, SELE-, 12(S)-HETE- and adhesion assays were performed to investigate the anti-metastatic properties of xanthohumol. Western blot analyses were used to elucidate the mechanisms inhibiting CCID formation. Xanthohumol inhibited the activity of CYP, SELE and NF-kB and consequently, the formation of CCIDs at low micromolar concentrations. More specifically, xanthohumol affected ICAM-1 expression and adherence of MCF-7 cells to LECs, which is a prerequisite for CCID formation. Furthermore, markers of epithelial-to-mesenchymal transition (EMT) and of cell mobility such as paxillin, MCL2 and S100A4 were suppressed by xanthohumol. Xanthohumol attenuated tumour cell-mediated defects at the lymphendothelial barrier and inhibited EMT-like effects thereby providing a mechanistic explanation for the anti-intravasative/anti-metastatic properties of xanthohumol.

    Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Antineoplastic Agents; Biomarkers, Tumor; Blotting, Western; Breast Neoplasms; Cell Adhesion; Cell Movement; Coculture Techniques; Cytochrome P-450 CYP1A1; Dose-Response Relationship, Drug; E-Selectin; Endothelial Cells; Epithelial-Mesenchymal Transition; Female; Flavonoids; HEK293 Cells; Humans; Intercellular Adhesion Molecule-1; MCF-7 Cells; Neoplasm Invasiveness; NF-kappa B; Propiophenones; Spheroids, Cellular; Transfection

2013