11-mercaptoundecanoic-acid and 2-mercaptoacetate

11-mercaptoundecanoic-acid has been researched along with 2-mercaptoacetate* in 1 studies

Other Studies

1 other study(ies) available for 11-mercaptoundecanoic-acid and 2-mercaptoacetate

Article	Year
Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, 2020, Apr-01, Volume: 210 DNA methylation is involved in the oncogenesis of head and neck squamous cell carcinoma and could be used for early detection of cancer to increase the chances of cure, but unfortunately diagnosis is usually made at late stages of the disease. In this work we developed genosensors to detect DNA methylation of the MGMT gene in head and neck cancer cell lines. The probe for MGMT promoter methylation was immobilized on gold electrodes modified with 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayers (SAM). Detection was performed with electrochemical impedance spectroscopy, with clear distinction between methylated and non-methylated DNA from head and neck cell lines. The genosensor is sensitive with a low detection limit of 0.24 × 10 Topics: Cell Line, Tumor; DNA Modification Methylases; DNA Repair Enzymes; Electrochemical Techniques; Electrodes; Fatty Acids; Gold; Head and Neck Neoplasms; Humans; Methylation; Promoter Regions, Genetic; Spectrophotometry, Infrared; Sulfhydryl Compounds; Thioglycolates; Tumor Suppressor Proteins	2020

Article

Year

Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines.

Talanta, 2020, Apr-01, Volume: 210

DNA methylation is involved in the oncogenesis of head and neck squamous cell carcinoma and could be used for early detection of cancer to increase the chances of cure, but unfortunately diagnosis is usually made at late stages of the disease. In this work we developed genosensors to detect DNA methylation of the MGMT gene in head and neck cancer cell lines. The probe for MGMT promoter methylation was immobilized on gold electrodes modified with 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayers (SAM). Detection was performed with electrochemical impedance spectroscopy, with clear distinction between methylated and non-methylated DNA from head and neck cell lines. The genosensor is sensitive with a low detection limit of 0.24 × 10

Topics: Cell Line, Tumor; DNA Modification Methylases; DNA Repair Enzymes; Electrochemical Techniques; Electrodes; Fatty Acids; Gold; Head and Neck Neoplasms; Humans; Methylation; Promoter Regions, Genetic; Spectrophotometry, Infrared; Sulfhydryl Compounds; Thioglycolates; Tumor Suppressor Proteins

2020