10-nitro-oleic-acid and cyclopentenone

10-nitro-oleic-acid has been researched along with cyclopentenone* in 1 studies

Other Studies

1 other study(ies) available for 10-nitro-oleic-acid and cyclopentenone

ArticleYear
Nitro-fatty acids and cyclopentenone prostaglandins share strategies to activate the Keap1-Nrf2 system: a study using green fluorescent protein transgenic zebrafish.
    Genes to cells : devoted to molecular & cellular mechanisms, 2011, Volume: 16, Issue:1

    Nitro-fatty acids are electrophilic fatty acids produced in vivo from nitrogen peroxide that have many physiological activities. We recently demonstrated that nitro-fatty acids activate the Keap1-Nrf2 system, which protects cells from damage owing to electrophilic or oxidative stresses via transactivating an array of cytoprotective genes, although the molecular mechanism how they activate Nrf2 is unclear. A number of chemical compounds with different structures have been reported to activate the Keap1-Nrf2 system, which can be categorized into at least six classes based on their sensing pathways. In this study, we showed that nitro-oleic acid (OA-NO₂), one of major nitro-fatty acids, activates Nrf2 in the same manner that of a cyclopentenone prostaglandin 15-deoxy-Δ(12,14) -prostaglandin J₂ (15d-PGJ₂) using transgenic zebrafish that expresses green fluorescent protein (GFP) in response to Nrf2 activators. In transgenic embryos, GFP was induced in the whole body by treatment with OA-NO₂, 15d-PGJ₂ or diethylmaleate (DEM), but not with hydrogen peroxide (H₂O₂), when exogenous Nrf2 and Keap1 were co-overexpressed. Induction by OA-NO₂ or 15d-PGJ₂ but not DEM was observed, even when a C151S mutation was introduced in Keap1. Our results support the contention that OA-NO₂ and 15d-PGJ₂ share an analogous cysteine code as electrophiles and also have similar anti-inflammatory roles.

    Topics: Animals; Animals, Genetically Modified; Carrier Proteins; Cell Line; Cells, Cultured; Cyclopentanes; Cysteine; Fatty Acids; Green Fluorescent Proteins; Hydrogen Peroxide; NF-E2-Related Factor 2; Oleic Acids; Oxidative Stress; Prostaglandin D2; Prostaglandins; Trans-Activators; Zebrafish; Zebrafish Proteins

2011