1-palmitoyl-2-oleoylphosphatidylethanolamine has been researched along with 1-2-dipalmitoylphosphatidylglycerol* in 2 studies
2 other study(ies) available for 1-palmitoyl-2-oleoylphosphatidylethanolamine and 1-2-dipalmitoylphosphatidylglycerol
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Interactions of oritavancin, a new semi-synthetic lipoglycopeptide, with lipids extracted from Staphylococcus aureus.
Oritavancin, a lipoglycopeptide with marked bactericidal activity against vancomycin-resistant Staphylococcus aureus and enterococci, induces calcein release from CL:POPE and POPG:POPE liposomes, an effect enhanced by an increase in POPG:POPE ratio, and decreased when replacing POPG by DPPG (Domenech et al., Biochim Biophys Acta 2009; 1788:1832-40). Using vesicles prepared from lipids extracted from S. aureus, we showed that oritavancin induces holes, erosion of the edges, and decrease of the thickness of the supported lipid bilayers (atomic force microscopy; AFM). Oritavancin also induced an increase of membrane permeability (calcein release) on a time- and dose-dependent manner. These effects were probably related to the ability of the drug to bind to lipid bilayers as shown by 8-anilino-1- naphthalene sulfonic acid (ANS) assay. Interaction of oritavancin with phospholipids at the level of their glycerol backbone and hydrophobic domain was studied by monitoring changes of Laurdan excitation generalized polarization (GP(ex)) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence anisotropy upon temperature increase. Oritavancin increased GP(ex) values and the transition temperature, indicating a more ordered structure at the level of the glycerol backbone. Oritavancin slightly decreased DPH fluorescence depolarization intensities, suggesting an increase in fluidity at the level of acyl chains. Together, our data confirm the interaction of oritavancin with lipids and the potential role of a rigidifying effect at the level of glycerol backbone for membrane permeabilization. This work shows how AFM and biophysical methods may help in characterizing drug-membrane interactions, and sheds further light on the mode of action of oritavancin. Topics: Anilino Naphthalenesulfonates; Anti-Bacterial Agents; Diphenylhexatriene; Fluorescence Polarization; Glycopeptides; Lipid Bilayers; Lipids; Lipoglycopeptides; Microscopy, Atomic Force; Permeability; Phosphatidylethanolamines; Phosphatidylglycerols; Protein Binding; Staphylococcus aureus; Time Factors; Unilamellar Liposomes | 2010 |
Discontinuous unbinding of lipid multibilayers.
We have observed a discontinuous unbinding transition of lipid bilayer stacks composed of phosphatidylethanolamine and phosphatidylglycerol using x-ray diffraction. The unbinding is reversible and coincides with the main (L(beta)-->L(alpha)) transition of the lipid mixture. Interbilayer interaction potentials deduced from the diffraction data reveal that the bilayers in the L(beta) phase are only weakly bound. The unbinding transition appears to be driven by an abrupt increase in steric repulsion resulting from increased thermal undulations of the bilayers upon entering the fluid L(alpha) phase. Topics: Lipid Bilayers; Liposomes; Models, Chemical; Phosphatidylethanolamines; Phosphatidylglycerols; X-Ray Diffraction | 2003 |