1-palmitoyl-2-oleoylglycero-3-phosphoglycerol and didodecyldimethylammonium

When a giant vesicle composed of POPC (rendered anionic with 5 mol % POPG) touches a giant POPC vesicle (rendered cationic with 5 mol % of DDAB), the two vesicles adhere strongly. When, however, low levels (0.1-2 mol %) of a perylene-substituted lipid are incorporated in to the bilayer, the vesicles separate at a rate that depends on the additive concentration. The vesicles that drift apart lose charge, indicating that the anionic and cationic components of the vesicles have interchanged upon contact. Presumably, the large perylene disrupts bilayer packing to allow the intervesicular exchange, and subsequent charge neutralization, to occur with up to 104 rate increases. It is possible that adhered living cells release one another by, similarly, producing low levels of a membrane-bound lipid or protein that induces so-called "kiss-and-run" vesicle events by promoting the release of adhesive elements.

Topics: Anions; Catalysis; Liposomes; Membrane Lipids; Phosphatidylcholines; Phosphatidylglycerols; Quaternary Ammonium Compounds