1-5-dihydro-fad and 9-10-phenanthrenequinone

1-5-dihydro-fad has been researched along with 9-10-phenanthrenequinone* in 1 studies

Other Studies

1 other study(ies) available for 1-5-dihydro-fad and 9-10-phenanthrenequinone

Article	Year
Effect of some redox mediators on FAD fluorescence of glucose oxidase from Penicillium adametzii LF F-2044.1. Enzyme and microbial technology, 2015, Volume: 72 Glucose oxidase (GOx) of Penicillium adametzii LF F-2044.1 recovered by ultrafiltration, was characterized by spectrophotometric and spectrofluorometric methods. It was shown that spectra of GOx from P. adametzii are typical for flavoproteins. Optimal buffer composition was chosen. It was determined that the GOx is the most efficiently interacting with substrate (glucose) in phosphate buffer at pH 7.0 with kkat/KM=15,217±550M(-1)s(-1). P. adametzii GOx fluorescence in the presence of different redox mediators (9,10-phenanthroline-5,6-dione, 9,10-phenanthrenequinone, 1,4-benzoquinone, methylene blue, ferrocene, ferrocenecarboxylic acid, α-methylferrocenemethanol, ferrocenecarboxaldehyde) was evaluated. Maximal differences in fluorescence emission intensity were observed in the presence of ferrocene and 9,10-phenanthrenequinone. Topics: Biotechnology; Ferrous Compounds; Flavin-Adenine Dinucleotide; Fungal Proteins; Glucose Oxidase; Kinetics; Metallocenes; Oxidation-Reduction; Penicillium; Phenanthrenes; Spectrometry, Fluorescence; Spectrophotometry	2015

Article

Year

Effect of some redox mediators on FAD fluorescence of glucose oxidase from Penicillium adametzii LF F-2044.1.

Enzyme and microbial technology, 2015, Volume: 72

Glucose oxidase (GOx) of Penicillium adametzii LF F-2044.1 recovered by ultrafiltration, was characterized by spectrophotometric and spectrofluorometric methods. It was shown that spectra of GOx from P. adametzii are typical for flavoproteins. Optimal buffer composition was chosen. It was determined that the GOx is the most efficiently interacting with substrate (glucose) in phosphate buffer at pH 7.0 with kkat/KM=15,217±550M(-1)s(-1). P. adametzii GOx fluorescence in the presence of different redox mediators (9,10-phenanthroline-5,6-dione, 9,10-phenanthrenequinone, 1,4-benzoquinone, methylene blue, ferrocene, ferrocenecarboxylic acid, α-methylferrocenemethanol, ferrocenecarboxaldehyde) was evaluated. Maximal differences in fluorescence emission intensity were observed in the presence of ferrocene and 9,10-phenanthrenequinone.

Topics: Biotechnology; Ferrous Compounds; Flavin-Adenine Dinucleotide; Fungal Proteins; Glucose Oxidase; Kinetics; Metallocenes; Oxidation-Reduction; Penicillium; Phenanthrenes; Spectrometry, Fluorescence; Spectrophotometry

2015