1-3-dimethylthiourea and dihydrorhodamine-123

1-3-dimethylthiourea has been researched along with dihydrorhodamine-123* in 1 studies

Other Studies

1 other study(ies) available for 1-3-dimethylthiourea and dihydrorhodamine-123

Article	Year
Oxidative stress during platelet-activating factor-induced microvascular dysfunction. Microcirculation (New York, N.Y. : 1994), 1996, Volume: 3, Issue:4 To assess the potential contribution of hydrogen peroxide (H2O2) to the leukocyte-endothelial cell adhesion and increased microvascular permeability (to fluorescein isothiocyanate [FITC]-albumin) observed in rat mesenteric venules exposed to platelet-activating factor (PAF).. The production of oxidants derived from H2O2 in mesenteric tissue was monitored using the H2O2-sensitive fluorochrome, dihydrorhodamine 123 (DHR). PAF elicited a rapid increase in both the albumin extravasation and oxidation of DHR, which was followed by an increased adherence and emigration of leukocytes in postcapillary venules.. The PAF-induced oxidation of DHR, leukocyte-endothelial cells interactions, and albumin leakage were attenuated by treatment with either catalase or dimethylthiourea. Treatment with monoclonal antibody directed against either CD11b/CD18 on leukocytes or ICAM-1 on endothelial cells attenuated the PAF-induced oxidative stress, albumin leakage, and leukocyte-endothelial cell adhesion.. These findings indicate that most of the oxidants generated in mesenteric tissue exposed to PAF results from the accumulation of activated leukocytes. Topics: Animals; Antibodies, Monoclonal; Capillary Permeability; Catalase; Cell Adhesion; Cell Adhesion Molecules; Fluorescein-5-isothiocyanate; Hydrogen Peroxide; Leukocytes; Male; Mesentery; Microcirculation; Microscopy, Video; Oxidation-Reduction; Oxidative Stress; Platelet Activating Factor; Rats; Rats, Sprague-Dawley; Rhodamines; Serum Albumin, Bovine; Thiourea	1996

Article

Year

Oxidative stress during platelet-activating factor-induced microvascular dysfunction.

Microcirculation (New York, N.Y. : 1994), 1996, Volume: 3, Issue:4

To assess the potential contribution of hydrogen peroxide (H2O2) to the leukocyte-endothelial cell adhesion and increased microvascular permeability (to fluorescein isothiocyanate [FITC]-albumin) observed in rat mesenteric venules exposed to platelet-activating factor (PAF).. The production of oxidants derived from H2O2 in mesenteric tissue was monitored using the H2O2-sensitive fluorochrome, dihydrorhodamine 123 (DHR). PAF elicited a rapid increase in both the albumin extravasation and oxidation of DHR, which was followed by an increased adherence and emigration of leukocytes in postcapillary venules.. The PAF-induced oxidation of DHR, leukocyte-endothelial cells interactions, and albumin leakage were attenuated by treatment with either catalase or dimethylthiourea. Treatment with monoclonal antibody directed against either CD11b/CD18 on leukocytes or ICAM-1 on endothelial cells attenuated the PAF-induced oxidative stress, albumin leakage, and leukocyte-endothelial cell adhesion.. These findings indicate that most of the oxidants generated in mesenteric tissue exposed to PAF results from the accumulation of activated leukocytes.

Topics: Animals; Antibodies, Monoclonal; Capillary Permeability; Catalase; Cell Adhesion; Cell Adhesion Molecules; Fluorescein-5-isothiocyanate; Hydrogen Peroxide; Leukocytes; Male; Mesentery; Microcirculation; Microscopy, Video; Oxidation-Reduction; Oxidative Stress; Platelet Activating Factor; Rats; Rats, Sprague-Dawley; Rhodamines; Serum Albumin, Bovine; Thiourea

1996