1-2-oleoylphosphatidylcholine and titanium-dioxide

The main aim of the study was to determine the effect of two polysaccharides: chitosan (Ch) and hyaluronic acid (HA), and/or titanium dioxide (TiO

Topics: Chitosan; Glycerylphosphorylcholine; Hyaluronic Acid; Phosphatidylcholines; Phospholipids; Titanium

TiO

Topics: Adsorption; Liposomes; Metal Nanoparticles; Methylamines; Oleic Acids; Phosphatidylcholines; Phosphatidylserines; Silicon Dioxide; Titanium

Lipid bilayers are biomembranes common to cellular life and constitute a continuous barrier between cells and their environment. Understanding the interaction of engineered nanomaterials (ENMs) with lipid bilayers is an important step toward predicting subsequent biological effects. In this study, we assess the effect of varying the surface functionality and concentration of 10-nm-diameter gold (Au) and titanium dioxide (TiO(2)) ENMs on the disruption of negatively charged lipid bilayer vesicles (liposomes) using a dye-leakage assay. Our findings show that Au ENMs having both positive and negative surface charge induce leakage that reaches a steady state after several hours. Positively charged particles with identical surface functionality and different core compositions show similar leakage effects and result in faster and greater leakage than negatively charged particles, which suggests that surface functionality, not particle core composition, is a critical factor in determining the interaction between ENMs and lipid bilayers. The results suggest that particles permanently adsorb to bilayers and that only one positively charged particle is required to disrupt a liposome and trigger the leakage of its entire contents in contrast to mellitin molecules, the most widely studied membrane lytic peptide, which requires hundred of molecules to generate leakage.

Topics: Cell Membrane; Engineering; Gold; Kinetics; Melitten; Nanoparticles; Particle Size; Phosphatidylcholines; Surface Properties; Titanium; Unilamellar Liposomes

Solid-state nanopore sensors are highly versatile platforms for the rapid, label-free electrical detection and analysis of single molecules, applicable to next generation DNA sequencing. The versatility of this technology allows for both large scale device integration and interfacing with biological systems. Here we report on the development of a hybrid biological solid-state nanopore platform that incorporates a highly mobile lipid bilayer on a single solid-state Al(2)O(3) nanopore sensor, for the potential reconstitution of ion channels and biological nanopores. Such a system seeks to combine the superior electrical, thermal, and mechanical stability of Al(2)O(3) solid-state nanopores with the chemical specificity of biological nanopores. Bilayers on Al(2)O(3) exhibit higher diffusivity than those formed on TiO(2) and SiO(2) substrates, attributed to the presence of a thick hydration layer on Al(2)O(3), a key requirement to preserving the biological functionality of reconstituted membrane proteins. Molecular dynamics simulations demonstrate that the electrostatic repulsion between the dipole of the DOPC headgroup and the positively charged Al(2)O(3) surface may be responsible for the enhanced thickness of this hydration layer. Lipid bilayer coated Al(2)O(3) nanopore sensors exhibit excellent electrical properties and enhanced mechanical stability (GΩ seals for over 50 h), making this technology ideal for use in ion channel electrophysiology, the screening of ion channel active drugs and future integration with biological nanopores such as α-hemolysin and MspA for rapid single molecule DNA sequencing. This technology can find broad application in bio-nanotechnology.

Topics: Aluminum Oxide; Biosensing Techniques; DNA; Fluorescence Recovery After Photobleaching; Ion Channels; Lipid Bilayers; Models, Biological; Molecular Dynamics Simulation; Nanopores; Nanotechnology; Phosphatidylcholines; Sequence Analysis, DNA; Silicon Dioxide; Staining and Labeling; Titanium

Molecular dynamics simulations are carried out to study the adsorption of three lipids, namely, DOPC, DOPS, and DMTAP, on TiO2(110) rutile surfaces and the influence of the interface on their conformational properties. Three types of rutile (110) surfaces, characterized by a different degree of hydroxylation (the neutral nonhydroxylated and hydroxylated surfaces and a partially hydroxylated surface with charge density corresponding to physiological pH) are investigated using force fields derived from ab initio calculations and experimental data. It is found that the stability of the adsorbate and the strength of the attachment are strictly connected with the nature of both the lipid and the surface. Direct coordination of the phosphate or carbonyl oxygens of the lipids with available titanium sites, observed in the case of partially or nonhydroxylated layers, determines stronger adsorption and, as a consequence, reduced dynamics. For a given hydration state of the surface, the adsorption strengths are in the order DOPS > DOPC >> DMTAP, in agreement with experimental data according to which the presence of DOPS units inside lipid bilayers favors stronger adsorption and lower mobility. The adsorption geometry, the hydration state of the lipid headgroups, and the dynamical processes (detachment, diffusion, etc.) occurring at the lipid/oxide interface are analyzed in detail, putting on a roughly quantitative basis time scales and energy barriers of the latter processes.

Topics: Adsorption; Chemistry, Physical; Computer Simulation; Diffusion; Lipid Bilayers; Lipids; Molecular Conformation; Myristates; Oxides; Phosphatidylcholines; Phosphatidylserines; Probability; Quaternary Ammonium Compounds; Solutions; Titanium

Supported phospholipid bilayers (SPBs) are useful for studying cell adhesion, cell-cell interactions, protein-lipid interactions, protein crystallization, and applications in biosensor and biomaterial areas. We have recently reported that SPBs could be formed on titanium dioxide, an important biomaterial, from vesicles containing anionic phospholipid phosphatidyl serine (PS) in the presence of calcium. Here, we show that the mobility of the fluorescently labeled PS present in these bilayers is severely restricted, whereas that of the zwitterionic phosphatidyl choline is not affected. Removal of calcium alleviated the restriction on the mobility of PS. Both components were found to be mobile in SPBs of identical compositions prepared in the presence of calcium on silica. To explain these results, we propose that, on TiO2, PS is trapped in the proximal leaflet of the bilayers. This proposal is supported by the results of protein adsorption experiments carried out on bilayers containing various amounts of PS prepared on silica and titania.

Topics: Adsorption; Calcium; Cell Adhesion; Cell Communication; Diffusion; Lipid Bilayers; Microscopy, Fluorescence; Phosphatidylcholines; Phosphatidylserines; Phospholipids; Silicon Dioxide; Spectrometry, Fluorescence; Temperature; Titanium