1-2-oleoylphosphatidylcholine has been researched along with 3-hydroxyflavone* in 2 studies
2 other study(ies) available for 1-2-oleoylphosphatidylcholine and 3-hydroxyflavone
| Article | Year |
|---|---|
Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes.
The dipole potential (Psi(d)) constitutes a large and functionally important part of the electrostatic potential of cell plasma membranes. However, its direct measurement is not possible. Herein, new 3-hydroxyflavone fluorescent probes were developed that respond strongly to Psi(d) changes by a variation of the intensity ratio of their two well-separated fluorescence bands. Using fluorescence spectroscopy with cell suspensions and confocal microscopy with adherent cells, we showed, for the first time, two-color fluorescence ratiometric measurement and visualization of Psi(d) in cell plasma membranes. Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced. Topics: Animals; Cell Adhesion; Cell Line; Cell Line, Tumor; Cell Membrane; Dose-Response Relationship, Drug; Fibroblasts; Flavonoids; Fluorescent Dyes; Humans; Hydrogen-Ion Concentration; Ketocholesterols; Lipid Bilayers; Lipids; Membrane Potentials; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Models, Chemical; Phosphatidylcholines; Phosphatidylglycerols; Protons; Pyridinium Compounds; Static Electricity | 2005 |
Novel two-band ratiometric fluorescence probes with different location and orientation in phospholipid membranes.
3-hydroxyflavone (3-HF) derivatives are very attractive fluorescence sensors due to their ability to respond to small changes in their microenvironment via a dramatic alteration of the relative intensities of their two well-separated emission bands. We developed fluorescence probes with locations at different depths and orientations of 3-HF moiety in the phospholipid bilayer, which determine their fluorescence behavior. While the spectral shifts of the probes correlate with their binding site polarity, the intensity ratio is a complex parameter that is also sensitive to the local hydration. We demonstrate that even the deeply located probes sense this hydration effect, which can be modulated by the charge of the lipid heads and is anisotropic with respect to the bilayer plane. Thus the two-band ratiometric fluorescence probes can provide multiparametric information on the properties of lipid membranes at different depths. Topics: Flavonoids; Fluorescent Dyes; Lipid Bilayers; Liposomes; Phosphatidylcholines; Phospholipids; Spectrometry, Fluorescence; Static Electricity; Water | 2002 |