1-2-dioleoyloxy-3-(trimethylammonium)propane has been researched along with fluorexon* in 2 studies
2 other study(ies) available for 1-2-dioleoyloxy-3-(trimethylammonium)propane and fluorexon
| Article | Year |
|---|---|
Cationic Nanoliposomes Are Efficiently Taken up by Alveolar Macrophages but Have Little Access to Dendritic Cells and Interstitial Macrophages in the Normal and CpG-Stimulated Lungs.
The purpose of this study was to assess whether cationic nanoliposomes could address tumor vaccines to dendritic cells in the lungs in vivo. Nanoliposomes were prepared using a cationic lipid, dimethylaminoethanecarbamoyl-cholesterol (DC-cholesterol) or dioleoyltrimethylammoniumpropane (DOTAP), and dipalmitoylphosphatidylcholine (DPPC), the most abundant phospholipid in lung surfactant. The liposomes presented a size below 175 nm and they effectively entrapped tumor antigens, an oligodeoxynucletotide containing CpG motifs (CpG) and the fluorescent dye calcein used as a tracer. Although the liposomes could permanently entrap a large fraction of the actives, they could not sustain their release in vitro. Liposomes made of DOTAP were safe to respiratory cells in vitro, while liposomes composed of DC-cholesterol were cytotoxic. DOTAP nanoliposomes were mainly taken up by alveolar macrophages following delivery to the lungs in mice. Few dendritic cells took up the liposomes, and interstitial macrophages did not take up liposomal calcein more than they took up soluble calcein. Stimulation of the innate immune system using liposomal CpG strongly enhanced uptake of calcein liposomes by all phagocytes in the lungs. Although a small percentage of dendritic cells took up the nanoliposomes, alveolar macrophages represented a major barrier to dendritic cell access in the lungs. Topics: 1,2-Dipalmitoylphosphatidylcholine; Adjuvants, Immunologic; Animals; Cancer Vaccines; Cell Line, Tumor; Cell Survival; Cholesterol; CpG Islands; Dendritic Cells; Drug Delivery Systems; Fatty Acids, Monounsaturated; Female; Fluoresceins; Fluorescent Dyes; gp100 Melanoma Antigen; Lipopeptides; Liposomes; Lung; Lung Neoplasms; Macrophages, Alveolar; MART-1 Antigen; Mice; Nanoparticles; Quaternary Ammonium Compounds; Tissue Distribution | 2019 |
Electrostatically mediated liposome fusion and lipid exchange with a nanoparticle-supported bilayer for control of surface charge, drug containment, and delivery.
The loading and containment of cargo within nanoparticles and their efficient delivery to cells represent a primary challenge in nanomedicine. We report lipid exchange between free and mesoporous silica nanoparticle-supported lipid bilayers as an effective means of containing cargo, controlling charge, and directing delivery to mammalian cells. The delivery of a membrane-impermeable dye (calcein) and a chemotherapeutic drug (doxorubicin) are demonstrated. Exchanged lipid bilayers minimized premature drug release, and an overall positive charge on the supported lipid bilayer effected enhanced delivery. Topics: Animals; CHO Cells; Cricetinae; Cricetulus; Doxorubicin; Drug Delivery Systems; Fatty Acids, Monounsaturated; Fluoresceins; Lipid Bilayers; Liposomes; Nanoparticles; Quaternary Ammonium Compounds; Silicon Dioxide; Spectrometry, Fluorescence; Static Electricity; Surface Properties | 2009 |