Compounds > 1-1-diphenyl-2-picrylhydrazyl

1-1-diphenyl-2-picrylhydrazyl and phycocyanobilin

1-1-diphenyl-2-picrylhydrazyl has been researched along with phycocyanobilin* in 1 studies

Other Studies

1 other study(ies) available for 1-1-diphenyl-2-picrylhydrazyl and phycocyanobilin

Article	Year
Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli. Biotechnology letters, 2013, Volume: 35, Issue:5 Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve ~3 mg PCB/l. A protocol for the purification of recombinant phycocyanobilin was established using solvent extraction combined with chromatography, which resulted in a final yield of ~0.3 mg PCB/l with a purity >95 %. Recombinant phycocyanobilin could scavenge hydroxyl radicals with an EC50 of 0.1 μM. Topics: Antioxidants; Biotechnology; Biphenyl Compounds; Chromatography, High Pressure Liquid; Escherichia coli; Phycobilins; Phycocyanin; Picrates; Spectrometry, Fluorescence	2013

Article

Year

Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli.

Biotechnology letters, 2013, Volume: 35, Issue:5

Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve ~3 mg PCB/l. A protocol for the purification of recombinant phycocyanobilin was established using solvent extraction combined with chromatography, which resulted in a final yield of ~0.3 mg PCB/l with a purity >95 %. Recombinant phycocyanobilin could scavenge hydroxyl radicals with an EC50 of 0.1 μM.

Topics: Antioxidants; Biotechnology; Biphenyl Compounds; Chromatography, High Pressure Liquid; Escherichia coli; Phycobilins; Phycocyanin; Picrates; Spectrometry, Fluorescence

2013