1-1-diphenyl-2-picrylhydrazyl and isoacteoside

Because of its good sensorial attributes, lemon verbena is used as a primary ingredient in infusions and nonalcoholic drinks. The present study was designed to assess the antioxidant activity (AA) of lemon verbena infusion (LVI) as well as the thermal stability of its AA and the content of polyphenolic compounds. The values reflecting the AA of LVI, including AA index, fast scavenging rate against 2,2-diphenyl-1-picrylhydrazyl, Trolox equivalent antioxidant capacity, and hydroxyl radical scavenging, are higher than those of many herbal infusions and antioxidant drinks estimated from reported data. In addition, the slope lag time and specific oxyradical antioxidant capacity values of LVI are comparable to those of a commercial antioxidant drink based on green tea. Hence, LVI is a source of bifunctional antioxidants, and thus in vivo studies of the antioxidant capacity of LVI would be useful to evaluate its potential as an ingredient in antioxidant drinks.

Topics: Antioxidants; Beverages; Biphenyl Compounds; Chromans; Chromatography, High Pressure Liquid; Flavonoids; Glucosides; Linear Models; Phenols; Picrates; Plant Extracts; Plant Leaves; Polyphenols; Reactive Oxygen Species; Spectrometry, Mass, Electrospray Ionization; Temperature; Verbena

The chromatographic separation of MeOH extract from Clerodendron trichotomum Thunberg leaves led to the isolation of three phenylpropanoid compounds. Using spectroscopic methods, the structures of these compounds were determined as beta-(3', 4'-dihydroxyphenyl)ethyl-O-alpha-L-rhamnopyranosyl (1-->3)-beta-D-(4-O-caffeoyl)-glucopyranoside, acteoside (verbascoside) (1), beta-(3', 4'-dihydroxyphenyl)ethyl-O-alpha-L-rhamnopyranosyl (1-->3)-beta-D-(6-O-caffeoyl)-glucopyranoside, isoacteoside (2), beta-(3', 4'-dihydroxyphenyl) ethyl-O-alpha-L-rhamnopyranosyl (1-->3)-beta-D-glucopyranoside, and decaffeoylacteoside (3). We measured the anti-inflammatory activity of these three phenylpropanoid compounds both in vitro (DPPH reduction assay, TBARS assay on Cu (2+)-induced oxidized LDL, PGE(2) assay) and in vivo (acetic acid induced vascular permeability in mice and carrageenan-induced hind paw edema in rats). 80% methanol fraction and acteoside had the activity.

Topics: Acetic Acid; Animals; Anti-Inflammatory Agents; Biphenyl Compounds; Capillary Permeability; Carrageenan; Catechols; Cell Line; Clerodendrum; Dinoprostone; Disaccharides; Disease Models, Animal; Dose-Response Relationship, Drug; Free Radical Scavengers; Glucosides; Humans; Inflammation; Magnetic Resonance Spectroscopy; Mast Cells; Mice; Molecular Structure; Phenols; Picrates; Plant Leaves; Rats; Thiobarbituric Acid Reactive Substances

A new cyclohexenone (1) and a new caffeoyl ester derivative (2), together with the known compounds (-)-isolariciresinol 3-alpha-O-beta-D-glucopyranoside (3), (+)-1-hydroxypinoresinol 1-O-beta-D-glucopyranoside (4), isoacteoside (5), luteolin 4'-O-beta-D-glucopyranoside (6), and indole-3-carboxylic acid (7), were isolated from the leaves of Bauhinia tarapotensis. The structures of these new compounds were determined by spectroscopic data analysis. The antioxidant activities of 1-7 were determined by measuring their free radical scavenging effects, using the 1,1-diphenyl-2-dipicrylhydrazyl free radical (DPPH) and Trolox equivalent antioxidant activity (TEAC) methods, and the coupled oxidation of beta-carotene and linoleic acid. Compounds 3-5 showed good activities in the DPPH and TEAC tests, while compounds 1 and 2 were active in the coupled oxidation of beta-carotene and linoleic acid bioassay.

Topics: Antioxidants; Benzothiazoles; Bepridil; Biphenyl Compounds; Caffeic Acids; Chromans; Chromatography, High Pressure Liquid; Cyclohexanones; Ecuador; Fabaceae; Flavonoids; Free Radical Scavengers; Glucosides; Indoles; Luteolin; Magnetic Resonance Spectroscopy; Molecular Structure; Phenols; Picrates; Plant Leaves; Plants, Medicinal; Sulfonic Acids