1-1-diphenyl-2-picrylhydrazyl has been researched along with hesperetin* in 6 studies
6 other study(ies) available for 1-1-diphenyl-2-picrylhydrazyl and hesperetin
Article | Year |
---|---|
Phytochemical analysis, antimicrobial, antioxidant activities and total phenols of Ferulago carduchorum in two vegetative stages (flower and fruit).
Ferulago carduchorum (Apiaceae family) is an endemic plant of Iran. The crude extract and four fractions of aerial parts of F. carduchorum in two vegetative stages (flower and fruit) were studied for their total phenolic contents, antimicrobial and antioxidant activities using folin-ciocalteu assay, micro dilution method and DPPH assay, respectively. The results indicated that the best antioxidant activity was determined in flower crude extract (IC50=0.44 mg/mL). The flower ethyl acetate fraction (FLE) showed better antimicrobial and antifungal activities than other fractions. So, FLE was selected for phytochemical investigations, resulting in isolation of a flavonoid (hesperetin). Hesperetin showed antimicrobial activity. The results showed that the antimicrobial and antioxidant effects during the flowering are obviously more than the fruit season. Topics: Anti-Infective Agents; Antioxidants; Apiaceae; Bacteria; Biphenyl Compounds; Flowers; Fruit; Fungi; Hesperidin; Microbial Sensitivity Tests; Phenols; Phytotherapy; Picrates; Plant Extracts; Plants, Medicinal; Solvents | 2016 |
Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey.
Two types of monofloral Malaysian honey (Gelam and Nenas) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation. Our results showed that both types of honey can scavenge free radicals and exhibit high antioxidant-reducing power; however, Gelam honey exhibited higher antioxidant activity (p < 0.05) than Nenas honey, which is in good correlation (r = 0.9899) with its phenolic contents. Interestingly, we also noted that both irradiated honeys have higher antioxidant activities and total phenolic and flavonoid contents compared to nonirradiated honeys by Folin-Ciocalteu and UV-spectrophotometry methods, respectively. However, HPLC analysis for phenolic compounds showed insignificant increase between irradiated and nonirradiated honeys. The phenolic compounds such as: caffeic acid, chlorogenic acid, ellagic acid, p- coumaric acid, quercetin and hesperetin as indicated by HPLC method were found to be higher in Gelam honey versus Nenas honey. In conclusion, irradiation of honey causes enhanced antioxidant activities and flavonoid compounds. Topics: Antioxidants; Biphenyl Compounds; Caffeic Acids; Chlorogenic Acid; Chromatography, High Pressure Liquid; Coumaric Acids; Ellagic Acid; Food Irradiation; Gamma Rays; Gastrointestinal Diseases; Hesperidin; Honey; Humans; Malaysia; Oxidation-Reduction; Picrates; Propionates; Quercetin; Spectrophotometry | 2011 |
Beneficial effect of hesperetin on cadmium induced oxidative stress in rats: an in vivo and in vitro study.
Cadmium (Cd) is ubiquitous in the environment and exposure through food and water as well as occupational sources can contribute to a well-defined spectrum of disease. The present study was undertaken to evaluate the role of hesperetin (Hp) in alleviating the Cd induced biochemical changes in rats.. During the experiment, male Wistar rats were injected with Cd 83 mg/kg day) subcutaneously alone or with oral administration of Hp 840 mg/kg day) for 21 days.. In Cd treated rats the levels of plasma lipid peroxidation (LPO) markers: thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) were significantly increased while the levels of plasma non-enzymatic antioxidants: reduced glutathione (GSH), vitamins C and E were significantly decreased in Cd administered rats. Administration of Hp along with Cd significantly decreased the level of LPO markers with elevation of non-enzymatic antioxidants in plasma. In vitro studies on the effect of Hp on scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH*), 2,2-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS+), superoxide anion (O*-), hydroxyl (OH*) radicals and reducing power also confirmed the free radical scavenging and antioxidant activity of Hp. In addition to that, ascorbic acid, butylated hydroxyl toluene was used as the reference antioxidant radical scavenger compounds. Thus, the observed effects are due to the free radical scavenging and antioxidant potential of Hp. Interestingly, among the different concentrations, tested 50 microM of Hp showed the highest antioxidant and free radical scavenging activities when compared to other concentrations.. The result of these findings provides further evidence to the neutraceutical and pharmaceutical potentials of Hp. Topics: Administration, Oral; Animals; Antioxidants; Ascorbic Acid; Benzothiazoles; Biomarkers; Biphenyl Compounds; Cadmium Chloride; Dose-Response Relationship, Drug; Environmental Pollutants; Glutathione; Hesperidin; Hydroxyl Radical; Injections, Subcutaneous; Lipid Peroxidation; Lipid Peroxides; Male; Oxidative Stress; Picrates; Rats; Rats, Wistar; Sulfonic Acids; Thiobarbituric Acid Reactive Substances; Vitamin E | 2011 |
Influence of L-rhamnosyl-D-glucosyl derivatives on properties and biological interaction of flavonoids.
The anti-proliferative activity of hesperetin, hesperidin, neohesperidin and rutin was evaluated on human hepatoma cell lines (Hep G2) and correlated to their antioxidant activity. The results obtained showed strong anti-proliferative effects of hesperidin and neohesperidin, considerably higher than the other two additives. Hesperetin induced caspase-3 activation, release of LDH and endogenous accumulation of putrescine. Cell cycle distribution seems to indicate that the inhibitory effects of polyphenols on cell growth could be due to G0/G1 block, and activation of apoptotic pathway in the presence of hesperetin. Our results underline also that the glycone forms show reduced scavenging activity against DPPH, but present a remarkable inhibition of cell proliferation and low cytotoxicity. Topics: Animals; Apoptosis; Biomarkers; Biphenyl Compounds; Caspase 3; Cell Line, Tumor; Cell Proliferation; Flavonoids; Free Radicals; Hesperidin; Humans; Picrates; Polyamines; Rutin | 2009 |
Antioxidant and neuroprotective effects of hesperidin and its aglycone hesperetin.
The present study evaluated antioxidant and neuroprotective activities of hesperidin, a flavanone mainly isolated from citrus fruits, and its aglycone hesperetin using cell-free bioassay system and primary cultured rat cortical cells. Both hesperidin and hesperetin exhibited similar patterns of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities. While hesperidin was inactive, hesperetin was found to be a potent antioxidant, inhibiting lipid peroxidation initiated in rat brain homogenates by Fe2+ and L-ascorbic acid. In consistence with these findings, hesperetin protected primary cultured cortical cells against the oxidative neuronal damage induced by H2O2 or xanthine and xanthine oxidase. In addition, it was shown to attenuate the excitotoxic neuronal damage induced by excess glutamate in the cortical cultures. When the excitotoxicity was induced by the glutamate receptor subtype-selective ligands, only the N-methyl-D-aspartic acid-induced toxicity was selectively and markedly inhibited by hesperetin. Furthermore, hesperetin protected cultured cells against the A(beta(25-35))-induced neuronal damage. Hesperidin, however, exerted minimal or no protective effects on the neuronal damage tested in this study. Taken together, these results demonstrate potent antioxidant and neuroprotective effects of hesperetin, implying its potential role in protecting neurons against various types of insults associated with many neurodegenerative diseases. Topics: Animals; Antioxidants; Biphenyl Compounds; Cell Survival; Cell-Free System; Cells, Cultured; Cerebral Cortex; Free Radical Scavengers; Hesperidin; Lipid Peroxidation; Neurons; Neuroprotective Agents; Oxidative Stress; Picrates; Rats | 2006 |
Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA.
Phenolic phytochemicals are thought to promote optimal health, partly via their antioxidant effects in protecting cellular components against free radicals. The aims of this study were to assess the free radical-scavenging activities of several common phenolic phytochemicals, and then, the effects of the most potent phenolic phytochemicals on oxidative damage to DNA in cultured cells. Epigallocatechin gallate (EGCG) scavenged the stable free radical, alpha,alpha-diphenyl-beta-picrylhydrazyl (DPPH), most effectively, while quercetin was about half as effective. Genistein, daidzein, hesperetin, and naringenin did not scavenge DPPH appreciably. Jurkat T-lymphocytes that were pre-incubated with relatively low concentrations of either EGCG or quercetin were less susceptible to DNA damage induced by either a reactive oxygen species or a reactive nitrogen species, as evaluated by the comet assay. More specifically, control cells had a comet score of only 17+/-5, indicating minimal DNA damage. Cells challenged with 25 microM hydrogen peroxide (H(2)O(2)) or 100 microM 3-morpholinosydnonimine (SIN-1, a peroxynitrite generator) had comet scores of 188+/-6 and 125+/-12, respectively, indicating extensive DNA damage. The H(2)O(2)-induced DNA damage was inhibited with 10 microM of either EGCG (comet score: 113+/-23) or quercetin (comet score: 82+/-7). Similarly, the SIN-1-mediated DNA damage was inhibited with 10 microM of either EGCG (comet score: 79+/-13) or quercetin (comet score: 72+/-17). In contrast, noticeable DNA damage was induced in Jurkat T-lymphocytes by incubating with 10-fold higher concentrations (i.e., 100 microM) of either EGCG (comet score: 56+/-17) or quercetin (comet score: 64+/-13) by themselves. Collectively, these data suggest that low concentrations of EGCG and quercetin scavenged free radicals, thereby inhibiting oxidative damage to cellular DNA. But, high concentrations of either EGCG or quercetin alone induced cellular DNA damage. Topics: Antioxidants; Bepridil; Biphenyl Compounds; Catechin; Comet Assay; DNA; DNA Damage; Flavanones; Flavonoids; Free Radical Scavengers; Genistein; Hesperidin; Humans; Indicators and Reagents; Isoflavones; Jurkat Cells; Molsidomine; Nitric Oxide Donors; Oxidation-Reduction; Picrates; Quercetin | 2000 |