1-1-diphenyl-2-picrylhydrazyl and cupric-chloride

We aimed in the reported study to investigate the impact of using various solvents in the extraction of potentially active compounds from Algerian propolis. Phenolic and flavonoids contents in association with antioxidant activity of the tested extracts were evaluated. Moreover phenolic composition was determined using UFLC-MS/MS. The tested parameters varied according to the used solvent. Total phenolic and flavononid contents ranged from 0.81±0.16 to 8.97±0.25 EGA mg/g and from 0.57±0.01 to 3.53±0.84 EQ mg/g respectively. All the investigated extracts demonstrated notable antiradical and reducing activities. Ethyl acetate and n-butanol were found to contain the highest amounts of phenolic and flavonoid compounds and the strongest antioxidant properties. The antioxidant activity of propolis extracts appears to be largely influenced by total phenolic and flavonoid contents. Rutin, chlorogenic, ferulic, caffeic and gallic acids were found to be the main phenolic compounds in Algerian propolis. Our results suggest that Algerian propolis may be a poplar-type propolis.

Topics: Algeria; Antioxidants; Benzothiazoles; Biphenyl Compounds; Chlorides; Chromatography, Liquid; Copper; Ferric Compounds; Hydroxybenzoates; Picrates; Propolis; Solvents; Spectrometry, Mass, Electrospray Ionization; Sulfonic Acids; Tandem Mass Spectrometry

Topics: Antimutagenic Agents; Antioxidants; Biphenyl Compounds; Copper; DNA, Bacterial; Dose-Response Relationship, Drug; Ferricyanides; Gas Chromatography-Mass Spectrometry; Molybdenum; Mutagenicity Tests; Mutagens; Mutation; Oxidation-Reduction; Phenols; Phytotherapy; Picrates; Plant Extracts; Plant Leaves; Plants, Medicinal; Psidium; Salmonella; Solvents

From the reaction of Cu(II) with the non-steroidal anti-inflammatory drug ketoprofen (Hketo), complex [Cu

Topics: 2,2'-Dipyridyl; Aminopyridines; Animals; Anti-Inflammatory Agents, Non-Steroidal; Benzothiazoles; Binding, Competitive; Biphenyl Compounds; Cattle; Coordination Complexes; Copper; Crystallography, X-Ray; DNA; Ethidium; Free Radical Scavengers; Hydroxyl Radical; Ketoprofen; Models, Molecular; Phenanthrolines; Picrates; Protein Binding; Serum Albumin; Sulfonic Acids

A series of six novel heterocyclic chalcone analogues 4(a-f) has been synthesized by condensing 2-acetyl-5-chlorothiophene with benzaldehyde derivatives in methanol at room temperature using a catalytic amount of sodium hydroxide. The newly synthesized compounds are characterized by IR, mass spectra, elemental analysis and melting point. Subsequently; the structures of these compounds were determined using single crystal X-ray diffraction. All the synthesized compounds were screened for their antioxidant potential by employing various in vitro models such as DPPH free radical scavenging assay, ABTS radical scavenging assay, ferric reducing antioxidant power and cupric ion reducing antioxidant capacity. Results reflect the structural impact on the antioxidant ability of the compounds. The IC₀ values illustrate the mild to good antioxidant activities of the reported compounds. Among them, 4f with a p-methoxy substituent was found to be more potent as antioxidant agent.

Topics: Benzothiazoles; Biphenyl Compounds; Chalcones; Copper; Crystallography, X-Ray; Ferricyanides; Free Radical Scavengers; Free Radicals; Models, Molecular; Molecular Conformation; Oxidation-Reduction; Picrates; Sulfonic Acids

Raspberry (Aksu Kirmizisi, Rubin, Newburgh, Hollanda Boduru, Heritage) and blackberry (Bursa 1, Bursa 2, Jumbo, Chester) cultivars were assayed for antioxidant activity (determined as 2,2-azino-di-[3-ethylbenzothialozine-sulphonic acid][ABTS], 2,2-diphenyl-1-picrylhydrazyl radical [DPPH], and cupric ion reducing antioxidant capacity [CUPRAC]), total phenol, total flavonoid, and total anthocyanin contents. In addition, 10 anthocyanins and anthocyanidins were determined in raspberry and blackberry by liquid chromatography-mass spectrometry (LC-MS/MS). Raspberry and blackberry had the highest ABTS, DPPH, CUPRAC, total phenol, and total flavonoid contents in methanol extracts, whereas total anthocyanin contents were the highest in water extracts. The antioxidant activity of the raspberry and blackberry was directly related to the total amount of phenolic compounds detected in the raspberry and blackberry. All antioxidant activity values were highly correlated with anthocyanin content in blackberry (0.93 < or = r < or = 0.99, P = 0.05). On the other hand, high correlation between total flavonoid content and antioxidant activity was recorded in water extract of blackberry (0.91 < or = r < or = 0.93, P = 0.05). ABTS value was highly correlated with total flavonoid content in methanol extract (r = 0.90), whereas total flavonoid content was relatively less correlated with DPPH (r = 0.85) and CUPRAC (r = 0.89).

Topics: Algorithms; Anthocyanins; Antioxidants; Benzothiazoles; Biphenyl Compounds; Chromatography, High Pressure Liquid; Copper; Flavonoids; Fruit; Molecular Weight; Phenols; Picrates; Plant Extracts; Rosaceae; Species Specificity; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry; Sulfonic Acids; Tandem Mass Spectrometry