1-1-diphenyl-2-picrylhydrazyl and caryophyllene

The chemical composition of the essential oil of

Topics: Biphenyl Compounds; Free Radical Scavengers; Gas Chromatography-Mass Spectrometry; Inhibitory Concentration 50; Malaysia; Monocyclic Sesquiterpenes; Myristicaceae; Oils, Volatile; Picrates; Polycyclic Sesquiterpenes; Sesquiterpenes, Germacrane

Topics: Acyclic Monoterpenes; Antioxidants; Benzothiazoles; Biphenyl Compounds; Gas Chromatography-Mass Spectrometry; Monoterpenes; Oils, Volatile; Picrates; Plant Extracts; Polycyclic Sesquiterpenes; Scutellaria; Sesquiterpenes; Sesquiterpenes, Germacrane; Sulfonic Acids; Uzbekistan

The present investigation was to evaluate the effects of essential oils of Wedelia chinensis (Osbeck) on free radicals and in vivo antioxidant properties.. Essential oils were extracted using hydro-distillation and compound analysis was performed by GC-MS analysis. Screening for inhibitory activity was conducted by DPPH and OH-scavenging assays. In addition an in vivo study was carried out in cell line implanted cancer bearing mice with assessment of levels of catalase, superoxide dismutase, glutathione peroxidase, lipid peroxidation, nitric oxide and reduced glutathione. Finally, lungs were dissected out for histopathology study of metastasis.. GC-MS analysis revealed the presence of carvocrol and trans-caryophyllene as the major compounds with 96% comparison with the Wilily and NBS libraries. The essential oil exhibited significant inhibition in DPPH free radical formation. Whereas reducing power and hydroxyl radical scavenging activity are dose dependent. When compared with the standard, it was found that the essential oil has more or less equal activity in scavenging free radicals produced. In the animal studies, the level of antioxidant enzymes catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione, were found to be increased in treated groups whereas lipid peroxidation and nitric oxide were reduced. Histopathology report also shows that the essential oil has a significant combating effect against cancer development.. In all the in vitro assays, a significant correlation existed between the concentrations of the essential oil and percentage inhibition of free radicals. The in vivo studies also has shown a very good antioxidant property for the essential oil during cancer development. From, these results the essential oil can be recommended for treating disease related to free radicals and to prevent cancer development.

Topics: Animals; Antioxidants; Biphenyl Compounds; Catalase; Cell Line, Tumor; Free Radical Scavengers; Free Radicals; Glutathione; Glutathione Peroxidase; Lipid Peroxidation; Lung Neoplasms; Male; Melanoma, Experimental; Mice; Mice, Inbred C57BL; Nitric Oxide; Oils, Volatile; Picrates; Plant Extracts; Plant Leaves; Polycyclic Sesquiterpenes; Sesquiterpenes; Superoxide Dismutase; Wedelia

The antioxidant activity of a commercial rectified clove leaf essential oil (Eugenia caryophyllus) and its main constituent eugenol was tested. This essential oil comprises in total 23 identified constituents, among them eugenol (76.8%), followed by beta-caryophyllene (17.4%), alpha-humulene (2.1%), and eugenyl acetate (1.2%) as the main components. The essential oil from clove demonstrated scavenging activity against the 2,2-diphenyl-1-picryl hydracyl (DPPH) radical at concentrations lower than the concentrations of eugenol, butylated hydroxytoluene (BHT), and butylated hydroxyanisole (BHA). This essential oil also showed a significant inhibitory effect against hydroxyl radicals and acted as an iron chelator. With respect to the lipid peroxidation, the inhibitory activity of clove oil determined using a linoleic acid emulsion system indicated a higher antioxidant activity than the standard BHT.

Topics: Antioxidants; Biphenyl Compounds; Butylated Hydroxyanisole; Butylated Hydroxytoluene; Eugenol; Free Radical Scavengers; Linoleic Acid; Monocyclic Sesquiterpenes; Oils, Volatile; Picrates; Plant Leaves; Polycyclic Sesquiterpenes; Sesquiterpenes; Syzygium; Thiobarbituric Acid Reactive Substances

In the present study, essential oil from the leaves of Syrian oreganum [Origanum syriacum L. (Lauraceae)] grown in Turkish state forests of the Dortyol district, Turkey, was obtained by steam distillation. The chemical composition of oil was analysed by GC and GC-MS, and was found to contain 49.02% monoterpenes, 36.60% oxygenated monoterpenes and 12.59% sesquiterpenes. The major components are as follows: gamma-terpinene, carvacrol, p-cymene and beta-caryophyllene. Subsequently, the reducing power, antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activities of the essential oil were studied. The reducing power was compared with ascorbic acid, and the other activities were compared with 2,6-di-tert-butyl-4-methyl phenol (BHT, butylated hydroxytoluene). The results showed that the activities were concentration dependent. The antioxidant activities of the oil were slightly lower than those of ascorbic acid or BHT, so the oil can be considered an effective natural antioxidant. Antimicrobial activities of the essential oil from the leaves of Origanum syriacum was also determined on 16 microorganisms tested using the agar-disc diffusion method, and showed antimicrobial activity against 13 of these.

Topics: Ampicillin; Anti-Infective Agents; Antioxidants; Ascorbic Acid; Biphenyl Compounds; Cyclohexane Monoterpenes; Cymenes; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Escherichia coli; Free Radical Scavengers; Klebsiella pneumoniae; Micrococcus luteus; Monoterpenes; Nystatin; Oils, Volatile; Origanum; Picrates; Plant Leaves; Plant Oils; Plants, Medicinal; Polycyclic Sesquiterpenes; Sesquiterpenes; Staphylococcus aureus; Streptomycin; Turkey