1-1-diphenyl-2-picrylhydrazyl and alpha-pinene

Chemical composition, antimicrobial and antioxidant activities of the essential oil of Ampelopsis megalophylla were evaluated in this research. GC-MS analysis of the essential oil revealed 42 compounds, representing 88.54% of the oil. The major compounds were borneol (10.81%), α-pinene (6.74%) and β-elemene (6.23%). The antimicrobial activity of the essential oil was evaluated against 13 micro-organisms using the disc diffusion and broth microdilution methods. Results demonstrated higher effects of this oil against Gram-positive bacteria than the other reference strains tested. The antioxidant effect of the essential oil was evaluated by using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,20-azinobis-3-ethylbenzthiazoline-6-sulfonate scavenging assays. The essential oil exhibited moderate antioxidant activity.

Topics: Ampelopsis; Anti-Bacterial Agents; Antioxidants; Bicyclic Monoterpenes; Biphenyl Compounds; Camphanes; Gas Chromatography-Mass Spectrometry; Gram-Positive Bacteria; Microbial Sensitivity Tests; Monoterpenes; Oils, Volatile; Picrates; Plant Leaves; Plant Oils; Sesquiterpenes

This study investigated the chemical composition (by using gas chromatography/flame ionization detection and gas chromatography/mass spectrometry, an antioxidant [1,1-diphenyl-2-picrylhydrazyl] [DPPH] radical-scavenging assay, and a 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonate [ABTS] radical cation-scavenging assay) and the antimalarial and cytotoxic activities of essential oil extracted from leaves of Melaleuca armillaris. Thirty-two components representing more than 98% of the total composition of the essential oil were identified. The main components were 1,8-cineole (85.8%), camphene (5.05%), and α-pinene (1.95%). The antioxidant activity by ABTS assay showed a mean (± standard deviation) 50% inhibitory concentration (IC(50)) value of 247.3 ± 3.9 mg/L, and the DPPH assay yielded an IC(50) value of 2183.6 ± 44.3 mg/L. The antimalarial study indicated that the essential oil had mild activity against the chloroquine-resistant Plasmodium falciparum FcB1 strain (IC(50), 27 ± 2 mg/L). The cytotoxic activity of this essential oil was tested against MCF7 human breast cancer cells and was found to be high (IC(50), 12 ± 1 mg/L).

Topics: Antimalarials; Antineoplastic Agents, Phytogenic; Antioxidants; Benzothiazoles; Bicyclic Monoterpenes; Biphenyl Compounds; Chromatography, Gas; Cyclohexanols; Eucalyptol; Flame Ionization; Gas Chromatography-Mass Spectrometry; Melaleuca; Monoterpenes; Oils, Volatile; Picrates; Plant Extracts; Plant Leaves; Sulfonic Acids; Terpenes

This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50-99.57% of the oils. Major components of the oils were α-pinene (58.61-77.39%), camphene (0.67-9.31%), δ-3-carene (0-10.01%) and trans-verbenol (0-5.24%). Antioxidant activities were determined by two different test systems, DPPH and ABTS radical scavenging activities. In both systems ripe berries exhibited better activity potential than the unripe ones.

Topics: Antioxidants; Bicyclic Monoterpenes; Biphenyl Compounds; Chromatography, Gas; Fruit; Gas Chromatography-Mass Spectrometry; Juniperus; Monoterpenes; Oils, Volatile; Picrates; Terpenes