1-1-diphenyl-2-picrylhydrazyl and 2-4-6-tripyridyl-s-triazine

The synthesis of derivatives of 4-Methylumbelliferone (4-MUs), which are structurally interesting antioxidants, was performed in this study. The modification of 4-Methylumbelliferone (4-MU) by different reaction steps was performed to yield the target compounds, the 4-MUs. The 4-MUs were characterized by different spectroscopic techniques (Fourier transform infrared; FT-IR and Nuclear magnetic resonance; NMR) and micro-elemental analysis (CHNS). The in vitro antioxidant activity of the 4-MUs was evaluated in terms of their free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH), Nitric oxide radical scavenging activity assay, chelating activity and their (FRAP) ferric-reducing antioxidant power, which were compared with a standard antioxidant. Our results reveal that the 4-MUs exhibit excellent radical scavenging activities. The antioxidant mechanisms of the 4-MUs were also studied. Density Function Theory (DFT)-based quantum chemical studies were performed with the basis set at 3-21G. Molecular models of the synthesized compounds were studied to understand the antioxidant activity. The electron levels, namely HOMO (highest occupied molecular orbital) and LUMO (lowest unoccupied molecular orbital), for these synthesized antioxidants were also studied.

Topics: Biphenyl Compounds; Cell Line, Tumor; Free Radical Scavengers; Humans; Hydrogen Peroxide; Hymecromone; Models, Molecular; Neoplasms; Nitric Oxide; Nuclear Magnetic Resonance, Biomolecular; Oxidation-Reduction; Picrates; Spectroscopy, Fourier Transform Infrared; Triazines

Dicranopteris linearis is a fern used traditionally for the treatment of skin afflictions such as external wounds, boils and ulcers. However, there are no scientific studies to date to demonstrate its ability to induce wound recovery. The objective of the present study was to explore the wound healing properties of an active fraction of D. linearis through several in vitro assays and to determine its chemical profile.. The antioxidant activities were evaluated by DPPH and FRAP assays. The ability of the active fraction to induce fibroblast cell proliferation in serum deprived medium or under the challenge of exogenous hydrogen peroxide was evaluated by MTT assay. Cell migration was investigated using the scratch-wound assay. Chemical constituents of the active fraction were isolated and their structures were determined by NMR and MS spectroscopic techniques. Chemical profiling was carried out by the HPLC-UV method.. The fifth subfraction of the methanol extract of D. linearis (sF5) showed prominent antioxidant activities. It was non-cytotoxic at the concentration tested and was able to induce cell proliferation of selected fibroblast cells under serum deprived conditions. In addition, sF5 was found to enhance cell migration and promote cellular repair following oxidative damage caused by hydrogen peroxide through a mechanism which was independent of its hydroxyl radical scavenging capability. Six phenolic glycosides were identified in sF5 where three were known, two were new and one phenolic glycoside first time reported from the plant.. The active fraction of D. linearis which was rich in polyphenolic substances exhibited substantial antioxidant activities, induced cellular repair and contributed positively to fibroblasts proliferation and migration in vitro. Hence, it may have potential application in inducing wound recovery and supports its ethnopharmacological use for treating skin problems.

Topics: Animals; Antioxidants; Biphenyl Compounds; Cell Line; Cell Movement; Cell Proliferation; Chlorides; Ferns; Ferric Compounds; Fibroblasts; Humans; Mice; Picrates; Plant Extracts; Plant Leaves; Polyphenols; Triazines; Wound Healing