1-1-diphenyl-2-picrylhydrazyl and 1-phenylpropanol

Blueberry (Vaccinium corymbosum) is a fruit very much appreciated by consumers for its antioxidant potential and health-promoting traits. Its beneficial potential properties are mainly due to a high content of anthocyanins and their amount can change after elicitation with methyl jasmonate. The aim of this work is to evaluate the changes in expression of several genes, accumulation of phenolic compounds and alterations in antioxidant potential in two different blueberry cultivars ('Duke' and 'Blueray') in response to methyl jasmonate (0.1 mM). Results showed that 9 h after treatment, the expression of phenylalanine ammonium lyase, chalcone synthase and anthocyanidin synthase genes was stimulated more in the 'Blueray' variety. Among the phenols measured an increase was recorded also for epicatechin and anthocyanin concentrations. 'Duke' is a richer sourche of anthocyanins compared to 'Blueray', treatment with methyl jasmonate promoted in 'Blueray' an increase in pigments as well as in the antioxidant potential, especially in fully ripe berries, but treated 'Duke' berries had greater levels, which were not induced by methyl jasmonate treatment. In conclusion, methyl jasmonate was, in some cases, an effective elicitor of phenolic metabolism and gene expression in blueberry, though with different intensity between cultivars.

Topics: Acetates; Anthocyanins; Biphenyl Compounds; Blueberry Plants; Carbohydrates; Cyclopentanes; Flavonols; Free Radical Scavengers; Gene Expression Regulation, Plant; Genes, Plant; Oxylipins; Phenols; Picrates; Plant Extracts; Propanols; Real-Time Polymerase Chain Reaction; RNA, Messenger; Stilbenes; Sucrose

Chemical investigation of the acetone extract from the aerial parts of the Mongolian medicinal plant Dracocephalum foetidum resulted in the isolation of three limonene glycosides, a caffeic acid trimer, four rosmarinic acid glucosides, and five acacetin acyl glycosides, together with 13 known natural products. The chemical structures of all of the compounds were determined by spectroscopic analyses. Among these compounds three showed hyaluronidase inhibitory activity. In addition, one other compound showed stronger 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity than the positive control Trolox, whereas three other compounds demonstrated a similar activity to that of Trolox.

Topics: Antioxidants; Biphenyl Compounds; Flavones; Glycosides; Hyaluronoglucosaminidase; Lamiaceae; Medicine, Mongolian Traditional; Mongolia; Monoterpenes; Picrates; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Propanols

By various chromatographic methods, one new phenylpropanoid glycoside, heterosmilaside (1), two known phenylpropanoid glycosides, helonioside B (2), and 2',6'-diacetyl-3,6-diferuloyl sucrose (3), and three known flavonoids, isoquercetin (4), quercetin-3-O-beta-D-glucuronopyranoside (5), and quercetin-3-O-(2''-alpha-L-rhamnopyranosyl)-beta-D-glucuronopyranoside (6) were isolated from the methanolic extract of the aerial part of Heterosmilax erythrantha Baill. Their structures were elucidated on the basis of spectroscopic analyses. All the isolated compounds were tested for antioxidant activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Among them, compounds 5 and 6 showed significant antioxidant activity with SC(50) values of 3.7 and 6.5 microg/mL, respectively.

Topics: Antioxidants; Biphenyl Compounds; Free Radical Scavengers; Glycosides; Molecular Structure; Picrates; Propanols; Smilacaceae