tetrazolium violet

Description

Tetrazolium violet is a redox indicator that is commonly used in cell viability assays. It is a colorless compound that is reduced to a purple formazan dye by living cells, allowing for the quantification of cell proliferation and viability. The dye is typically used in conjunction with other dyes, such as MTT or XTT, to assess cell viability. Tetrazolium violet is a safe and reliable reagent for cell viability assays. It has been shown to be effective in a variety of cell types, including cancer cells, bacteria, and fungi. Tetrazolium violet is a widely used reagent in research and is particularly useful for studying cell proliferation, cell death, and drug efficacy. The compound is typically synthesized through a multi-step process that involves the reaction of aromatic amines with diazonium salts. The mechanism of action of tetrazolium violet involves the reduction of the tetrazolium ring by electron-donating molecules, such as NADH and NADPH, which are found in living cells. This reduction results in the formation of a purple formazan dye, which can be measured spectrophotometrically. The amount of formazan dye produced is directly proportional to the number of living cells in the sample. Tetrazolium violet is often used in conjunction with other assays, such as the MTT assay and the XTT assay, to assess cell viability. These assays are typically used to determine the cytotoxic effects of drugs, chemicals, or other agents on cells. Tetrazolium violet is a valuable tool for research in a variety of fields, including cancer biology, toxicology, and drug discovery.'

tetrazolium violet: structure in first source [Medical Subject Headings (MeSH), National Library of Medicine, extracted Dec-2023]

tetrazolium violet : An organic chloride salt having tetrazolium violet(1+) as the counterion. [Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]