2-((4-bromo-2,3-dioxobutyl)thio)-1,n(6)-ethenoadenosine 2',5'-bisphosphate

Description

## 2-((4-bromo-2,3-dioxobutyl)thio)-1,n(6)-ethenoadenosine 2',5'-bisphosphate: A Powerful Tool for Studying Adenosine Kinase

The compound you've described, **2-((4-bromo-2,3-dioxobutyl)thio)-1,n(6)-ethenoadenosine 2',5'-bisphosphate**, is a complex molecule with several important features:

* **Ethenoadenosine:** The core of the molecule is an adenosine derivative with an etheno bridge connecting the N1 and N6 positions. This etheno bridge **increases the fluorescence of the molecule**, making it a useful probe for various biological studies.
* **2',5'-Bisphosphate:** The molecule contains two phosphate groups attached to the 2' and 5' positions of the ribose sugar. This allows the compound to **mimic the natural substrate of adenosine kinase, adenosine monophosphate (AMP).**
* **(4-bromo-2,3-dioxobutyl)thio:** This side chain is critical for the compound's function. It **inhibits the enzyme adenosine kinase** by acting as a **suicide substrate**, meaning it gets incorporated into the enzyme's active site and irreversibly disables it.

**Importance for Research:**

This compound is a **valuable tool for studying adenosine kinase**, a crucial enzyme involved in **purine metabolism and cellular signaling**. It allows researchers to:

* **Inhibit adenosine kinase activity**: The compound can effectively block the enzyme's function, enabling researchers to investigate the consequences of adenosine kinase inhibition in different biological contexts.
* **Investigate adenosine kinase's role in disease**: By studying the effects of adenosine kinase inhibition, researchers can gain insights into its role in various diseases, including cancer, inflammation, and neurological disorders.
* **Develop new drugs targeting adenosine kinase**: Understanding the mechanism of action of this compound can lead to the development of new drugs that specifically target adenosine kinase and potentially offer therapeutic benefits for specific diseases.
* **Track adenosine kinase activity**: The compound's fluorescent properties can be used to track the enzyme's activity in living cells, providing valuable insights into its localization, dynamics, and interactions with other cellular components.

**In summary**, 2-((4-bromo-2,3-dioxobutyl)thio)-1,n(6)-ethenoadenosine 2',5'-bisphosphate is a unique and powerful tool that has significantly contributed to our understanding of adenosine kinase, opening new avenues for research and therapeutic development.

2-((4-bromo-2,3-dioxobutyl)thio)-1,N(6)-ethenoadenosine 2',5'-bisphosphate: structure given in first source [Medical Subject Headings (MeSH), National Library of Medicine, extracted Dec-2023]