1-palmitoyl-2-(10-(4-((trifluoromethyl)diazirinyl)phenyl)-8-oxadecanoyl)-sn-glycero-3-phosphocholine

Description

1-palmitoyl-2-(10-(4-((trifluoromethyl)diazirinyl)phenyl)-8-oxadecanoyl)-sn-glycero-3-phosphocholine, also known as **1-palmitoyl-2-[(10-(4-trifluoromethyldiazirinyl)phenyl)-8-oxadecanoyl]-sn-glycero-3-phosphocholine** or **TriFM-DOPE**, is a **photoactivatable lipid analog**.

Here's a breakdown:

**Components:**

* **1-palmitoyl-2-[(10-(4-trifluoromethyldiazirinyl)phenyl)-8-oxadecanoyl]-sn-glycero-3-phosphocholine:** This is the full chemical name.
* **TriFM-DOPE:** This is a shorter, more commonly used name. It stands for TrifluoroMethyl-Diazirine-Octadecyl-PhosphoEthanolamine.
* **sn-glycero-3-phosphocholine:** This is the core structure of a phospholipid, a major component of cell membranes.
* **Palmitoyl:** This is a saturated fatty acid chain with 16 carbons.
* **(10-(4-((trifluoromethyl)diazirinyl)phenyl)-8-oxadecanoyl):** This is a modified fatty acid chain containing a diazirine group attached to a phenyl ring.

**Photoactivatable Property:**

* **Diazirine group:** This group is the key to the photoactivation. It is a highly reactive molecule that can be induced to form a **carbene** (a highly reactive species) when exposed to ultraviolet (UV) light.
* **Carbene:** This carbene will then react with and covalently bind to nearby molecules.

**Importance in Research:**

TriFM-DOPE is a valuable tool for studying **protein-lipid interactions** within cell membranes. This is because:

1. **Membrane Incorporation:** It can be incorporated into cell membranes due to its phospholipid structure.
2. **Specific Targeting:** The diazirine group allows for **targeted photolabeling** of proteins that are in close proximity to the lipid.
3. **Identification of Interactions:** After photoactivation, the covalent bond between the carbene and the target protein allows for **identification and analysis of the interacting proteins**.

**Applications in Research:**

* **Membrane protein studies:** TriFM-DOPE has been used to study the interaction of proteins with membrane lipids, revealing information about protein structure, function, and localization within the membrane.
* **Signal transduction research:** It helps understand how lipids are involved in signal transduction pathways and how proteins interact with lipids in these processes.
* **Drug discovery:** TriFM-DOPE can be used to identify potential drug targets that interact with specific lipids in the cell membrane.

**In summary, TriFM-DOPE is a powerful tool for studying protein-lipid interactions, offering valuable insights into cell membrane biology and facilitating research in various fields.**

1-palmitoyl-2-(10-(4-((trifluoromethyl)diazirinyl)phenyl)-8-oxadecanoyl)-sn-glycero-3-phosphocholine: RN given refers to parent cpd; RN given from first source [Medical Subject Headings (MeSH), National Library of Medicine, extracted Dec-2023]