1,n(6)-ethenoadenosine 3',5'-monophosphate

Description

You're likely referring to **1,N(6)-ethenoadenosine 3',5'-monophosphate (ε-Ado-3',5'-MP)**. This is a modified nucleotide, specifically a derivative of adenosine monophosphate (AMP), that has been extensively studied in biological and chemical research.

Here's a breakdown of the molecule and its importance:

**Structure and Properties:**

* **ε-Ado-3',5'-MP** is formed by the covalent attachment of a vinyl group (CH=CH₂) to the N6 and 1 positions of the adenine base in AMP.
* This modification adds a fluorescent property to the molecule. The fluorescence intensity is sensitive to changes in its environment, making it a useful probe in various research areas.

**Importance in Research:**

1. **Probing Nucleic Acid Structure and Dynamics:**
* The fluorescent properties of ε-Ado-3',5'-MP make it a valuable tool for studying nucleic acid structure and dynamics. It can be incorporated into DNA or RNA strands, and its fluorescence can be used to track changes in conformation or interaction with other molecules.

2. **Enzyme Activity Assays:**
* ε-Ado-3',5'-MP has been used to study the activity of various enzymes that act on nucleotides, such as kinases, phosphatases, and polymerases. Its fluorescence can be used to monitor enzymatic reactions, providing information about reaction rates and kinetics.

3. **Cell Signaling Studies:**
* Since ε-Ado-3',5'-MP is a derivative of cAMP, it can be used as a probe for studying the cAMP signaling pathway. It can be used to monitor cAMP levels in cells and tissues, providing insights into cellular signaling events.

4. **Drug Development:**
* The unique properties of ε-Ado-3',5'-MP have made it a useful tool in drug development. Its fluorescence can be used to screen for new drugs that interact with nucleic acids or enzymes.

**Examples of Research Applications:**

* **Study of DNA conformation:** ε-Ado-3',5'-MP has been used to monitor changes in DNA structure upon binding to proteins or other molecules.
* **Development of new fluorescent probes:** Researchers are developing new fluorescent probes based on ε-Ado-3',5'-MP for specific applications, such as tracking specific nucleic acid sequences in living cells.
* **Studies of the cAMP signaling pathway:** ε-Ado-3',5'-MP has been used to investigate the role of cAMP in various cellular processes, including cell growth, differentiation, and metabolism.

**In summary, 1,N(6)-ethenoadenosine 3',5'-monophosphate (ε-Ado-3',5'-MP) is a valuable tool for studying nucleic acid structure and dynamics, enzymatic activity, cell signaling, and drug development. Its fluorescent properties make it a versatile probe for various research applications.**

1,N(6)-ethenoadenosine 3',5'-monophosphate: fluorescent cyclic AMP analog; structure [Medical Subject Headings (MeSH), National Library of Medicine, extracted Dec-2023]