Compounds > 1,3-diethyl-2-thiobarbituric acid
Page last updated: 2024-12-07

1,3-diethyl-2-thiobarbituric acid

Description Research Excerpts Clinical Trials Roles Classes Pathways Study Profile Bioassays Related Drugs Related Conditions Protein Interactions Research Growth Market Indicators

Description

1,3-Diethyl-2-thiobarbituric acid (DTB) is a chemical compound that is used in research to measure the level of lipid peroxidation, a process that occurs when lipids are damaged by free radicals.

Here's a breakdown of its significance in research:

**What it is:**

* **Structure:** DTB is a heterocyclic compound containing a thiobarbituric acid ring, which is modified with two ethyl groups attached at positions 1 and 3.
* **Reactivity:** DTB reacts with malondialdehyde (MDA), a major product of lipid peroxidation. This reaction forms a colored complex that can be measured spectrophotometrically.

**Why it's important in research:**

* **Lipid peroxidation marker:** DTB is a widely used marker for lipid peroxidation, a process that can damage cell membranes, proteins, and DNA, leading to various diseases and conditions.
* **Assessing oxidative stress:** Lipid peroxidation is a major component of oxidative stress, which is linked to aging, cancer, cardiovascular disease, and neurodegenerative disorders.
* **Monitoring experimental interventions:** Researchers use DTB to monitor the effectiveness of antioxidants, anti-inflammatory agents, and other interventions aimed at reducing oxidative stress and lipid peroxidation.
* **Understanding disease mechanisms:** DTB helps scientists understand the role of lipid peroxidation in various diseases and develop new therapeutic strategies.

**Limitations:**

* **Specificity:** DTB can react with other compounds besides MDA, leading to potential overestimation of lipid peroxidation.
* **Sensitivity:** The DTB assay may not be sensitive enough to detect low levels of lipid peroxidation.

**Conclusion:**

1,3-Diethyl-2-thiobarbituric acid (DTB) is a valuable tool for researchers studying oxidative stress and lipid peroxidation. It provides a convenient and relatively straightforward method to quantify lipid peroxidation levels in various biological samples. While it has some limitations, DTB remains a widely used marker in a broad range of research areas.

1,3-diethyl-2-thiobarbituric acid: used in urinary test for smoking [Medical Subject Headings (MeSH), National Library of Medicine, extracted Dec-2023]

Cross-References

ID SourceID
PubMed CID78890
CHEMBL ID2136061
SCHEMBL ID1033205
MeSH IDM0133620

Synonyms (44)

Synonym
EN300-18198
bxb40m6a7w ,
unii-bxb40m6a7w
1,3-diethyl-2-thioxodihydropyrimidine-4,6(1h,5h)-dione
nsc158284
barbituric acid,3-diethyl-2-thio-
5217-47-0
mls003106467 ,
1,3-diethyl-2-thiobarbituric acid
n,n'-diethyl-2-thiobarbituric acid
n,n'-diethylthiobarbituric acid
nsc-158284
1,3-diethylthiobarbituric acid
4,5h)-pyrimidinedione, 1,3-diethyldihydro-2-thioxo-
1,3-diethyl-2-thiobarbituric acid, 99%
4,6(1h,5h)-pyrimidinedione, 1,3-diethyldihydro-2-thioxo-
1,3-diethyldihydro-2-thioxopyrimidine-4,6(1h,5h)-dione
nsc 158284
einecs 226-010-7
barbituric acid, 1,3-diethyl-2-thio-
inchi=1/c8h12n2o2s/c1-3-9-6(11)5-7(12)10(4-2)8(9)13/h3-5h2,1-2h3
shbtugjakbrbbj-uhfffaoysa-
smr001821370
1,3-diethyl-2-sulfanylidene-1,3-diazinane-4,6-dione
AKOS000119446
STK201353
1u6 ,
SCHEMBL1033205
CHEMBL2136061
1,3-diethyl-2-thioxodihydro-4,6(1h,5h)-pyrimidinedione #
DTXSID0022222
J-803024
3-[3-(piperidin-1-ylmethyl)pheno xy]propan-1-amine oxalic acid salt
SR-01000311815-1
sr-01000311815
mfcd00006676
Z57354986
1,3-diethyl-2-thiobarbituricacid
CS-0204337
1.3-diethyl-2-thiobarbituric acid
Q27274941
SB58150
BS-28659
F87998
[information is derived through text-mining from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Protein Targets (3)

Potency Measurements

ProteinTaxonomyMeasurementAverage (µ)Min (ref.)Avg (ref.)Max (ref.)Bioassay(s)
WRNHomo sapiens (human)Potency56.23410.168331.2583100.0000AID651768
hypothetical protein, conservedTrypanosoma bruceiPotency31.62280.223911.245135.4813AID624173
serine/threonine-protein kinase PLK1Homo sapiens (human)Potency6.70160.168316.404067.0158AID720504
[prepared from compound, protein, and bioassay information from National Library of Medicine (NLM), extracted Dec-2023]

Bioassays (10)

Assay IDTitleYearJournalArticle
AID588497High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain F protease, MLPCN compound set2010Current protocols in cytometry, Oct, Volume: Chapter 13Microsphere-based flow cytometry protease assays for use in protease activity detection and high-throughput screening.
AID588497High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain F protease, MLPCN compound set2006Cytometry. Part A : the journal of the International Society for Analytical Cytology, May, Volume: 69, Issue:5
Microsphere-based protease assays and screening application for lethal factor and factor Xa.
AID588497High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain F protease, MLPCN compound set2010Assay and drug development technologies, Feb, Volume: 8, Issue:1
High-throughput multiplex flow cytometry screening for botulinum neurotoxin type a light chain protease inhibitors.
AID588501High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Lethal Factor Protease, MLPCN compound set2010Current protocols in cytometry, Oct, Volume: Chapter 13Microsphere-based flow cytometry protease assays for use in protease activity detection and high-throughput screening.
AID588501High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Lethal Factor Protease, MLPCN compound set2006Cytometry. Part A : the journal of the International Society for Analytical Cytology, May, Volume: 69, Issue:5
Microsphere-based protease assays and screening application for lethal factor and factor Xa.
AID588501High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Lethal Factor Protease, MLPCN compound set2010Assay and drug development technologies, Feb, Volume: 8, Issue:1
High-throughput multiplex flow cytometry screening for botulinum neurotoxin type a light chain protease inhibitors.
AID651635Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expression
AID588499High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain A protease, MLPCN compound set2010Current protocols in cytometry, Oct, Volume: Chapter 13Microsphere-based flow cytometry protease assays for use in protease activity detection and high-throughput screening.
AID588499High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain A protease, MLPCN compound set2006Cytometry. Part A : the journal of the International Society for Analytical Cytology, May, Volume: 69, Issue:5
Microsphere-based protease assays and screening application for lethal factor and factor Xa.
AID588499High-throughput multiplex microsphere screening for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain A protease, MLPCN compound set2010Assay and drug development technologies, Feb, Volume: 8, Issue:1
High-throughput multiplex flow cytometry screening for botulinum neurotoxin type a light chain protease inhibitors.
[information is prepared from bioassay data collected from National Library of Medicine (NLM), extracted Dec-2023]

Research

Studies (14)

TimeframeStudies, This Drug (%)All Drugs %
pre-19902 (14.29)18.7374
1990's4 (28.57)18.2507
2000's2 (14.29)29.6817
2010's5 (35.71)24.3611
2020's1 (7.14)2.80
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Market Indicators

Research Demand Index: 19.42

According to the monthly volume, diversity, and competition of internet searches for this compound, as well the volume and growth of publications, there is estimated to be moderate demand-to-supply ratio for research on this compound.

MetricThis Compound (vs All)
Research Demand Index19.42 (24.57)
Research Supply Index2.77 (2.92)
Research Growth Index4.57 (4.65)
Search Engine Demand Index15.26 (26.88)
Search Engine Supply Index2.00 (0.95)

This Compound (19.42)

All Compounds (24.57)

Study Types

Publication TypeThis drug (%)All Drugs (%)
Trials0 (0.00%)5.53%
Reviews0 (0.00%)6.00%
Case Studies0 (0.00%)4.05%
Observational0 (0.00%)0.25%
Other15 (100.00%)84.16%
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]
SubstanceRelationship StrengthStudiesTrialsClassesRoles
chlorine
chloride : A halide anion formed when chlorine picks up an electron to form an an anion.		1.9810halide anion;
monoatomic chlorine		cofactor;
Escherichia coli metabolite;
human metabolite
phosphoric acid
phosphoric acid: concise etchant is 37% H3PO4. phosphoric acid : A phosphorus oxoacid that consists of one oxo and three hydroxy groups joined covalently to a central phosphorus atom.		1.9810phosphoric acidsalgal metabolite;
fertilizer;
human metabolite;
NMR chemical shift reference compound;
solvent
toluene
methylbenzene : Any alkylbenzene that is benzene substituted with one or more methyl groups.		2.0710methylbenzene;
toluenes;
volatile organic compound		cholinergic antagonist;
fuel additive;
neurotoxin;
non-polar solvent
camphor, (+-)-isomer
[no description available]		2.4820bornane monoterpenoid;
cyclic monoterpene ketone		plant metabolite
4-aminobenzoic acid
para-Aminobenzoates: Benzoic acids, salts, or esters that contain an amino group attached to carbon number 4 of the benzene ring structure.. 4-aminobenzoate : An aromatic amino-acid anion that is the conjugate base of 4-aminobenzoic acid.		2.0710aminobenzoate;
aromatic amino-acid anion		Escherichia coli metabolite;
plant metabolite;
Saccharomyces cerevisiae metabolite
methylene blue
Methylene Blue: A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN.. methylene blue : An organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties.		2.0510organic chloride saltacid-base indicator;
antidepressant;
antimalarial;
antimicrobial agent;
antioxidant;
cardioprotective agent;
EC 1.4.3.4 (monoamine oxidase) inhibitor;
EC 3.1.1.8 (cholinesterase) inhibitor;
EC 4.6.1.2 (guanylate cyclase) inhibitor;
fluorochrome;
histological dye;
neuroprotective agent;
physical tracer
barbituric acid
barbituric acid: RN given refers to parent cpd; structure from Merck Index, 9th ed, #966. barbituric acid : A barbiturate, the structure of which is that of perhydropyrimidine substituted at C-2, -4 and -6 by oxo groups. Barbituric acid is the parent compound of barbiturate drugs, although it is not itself pharmacologically active.		2.3720barbituratesallergen;
xenobiotic
triethylene glycol dimethacrylate
[no description available]		2.0710
thioxanthone
thioxanthone: structure in first source		2.0710
4-toluenesulfinic acid
4-toluenesulfinic acid: RN given refers to parent cpd		2.0710
malondialdehyde
Malondialdehyde: The dialdehyde of malonic acid.. malonaldehyde : A dialdehyde that is propane substituted by two oxo groups at the terminal carbon atoms respectively. A biomarker of oxidative damage to lipids caused by smoking, it exists in vivo mainly in the enol form.		7.3920dialdehydebiomarker
diphenyliodonium
diphenyliodonium: RN given refers to the parent coumpound; inhibitor of neutrophil superoxide generating oxidase; structure has been determined		2.0710
hydroxyethyl methacrylate
hydroxyethyl methacrylate: many of cited refs are for gel which refers to polymeric form of above cpd: POLYHYDROXYETHYL METHACRYLATE. 2-hydroxyethyl methacrylate : An enoate ester that is the monomethacryloyl derivative of ethylene glycol.		2.0710enoate esterallergen;
polymerisation monomer
bisphenol a-glycidyl methacrylate
Bisphenol A-Glycidyl Methacrylate: The reaction product of bisphenol A and glycidyl methacrylate that undergoes polymerization when exposed to ultraviolet light or mixed with a catalyst. It is used as a bond implant material and as the resin component of dental sealants and composite restorative materials.		2.0710diarylmethane
ferric chloride
ferric chloride: RN given refers to cpd with MF of Fe-Cl3; used to induce experimental arterial thrombosis to evaluate antithrombotic agents		1.9810iron coordination entityastringent;
Lewis acid
sodium selenite
disodium selenite : An inorganic sodium salt composed of sodium and selenite ions in a 2:1 ratio.		2.0510inorganic sodium salt;
selenite salt		nutraceutical
ethyl 4-dimethylaminobenzoate
ethyl 4-dimethylaminobenzoate: slow-acting amine polymerization accelerator. parbenate : A benzoate ester that is ethyl benzoate substituted by a dimethylamino group at position 4.		2.0710benzoate ester;
ethyl ester;
tertiary amino compound
camphoroquinone
camphoroquinone: a natural monoterpene, non-aromatic quinone; structure given in first source. bornane-2,3-dione : A bornane monoterpenoid that is bicyclo[2.2.1]heptane substituted by methyl groups at positions 1, 7 and 7 and oxo groups at positions 2 and 3.		2.4820bornane monoterpenoid;
carbobicyclic compound
nicotine
(S)-nicotine : A 3-(1-methylpyrrolidin-2-yl)pyridine in which the chiral centre has S-configuration. The naturally occurring and most active enantiomer of nicotine, isolated from Nicotiana tabacum.		7.89403-(1-methylpyrrolidin-2-yl)pyridineanxiolytic drug;
biomarker;
immunomodulator;
mitogen;
neurotoxin;
nicotinic acetylcholine receptor agonist;
peripheral nervous system drug;
phytogenic insecticide;
plant metabolite;
psychotropic drug;
teratogenic agent;
xenobiotic
cotinine
Cotinine: The N-glucuronide conjugate of cotinine is a major urinary metabolite of NICOTINE. It thus serves as a biomarker of exposure to tobacco SMOKING. It has CNS stimulating properties.. (-)-cotinine : An N-alkylpyrrolidine that consists of N-methylpyrrolidinone bearing a pyridin-3-yl substituent at position C-5 (the 5S-enantiomer). It is an alkaloid commonly found in Nicotiana tabacum.		2.420N-alkylpyrrolidine;
pyridines;
pyrrolidin-2-ones;
pyrrolidine alkaloid		antidepressant;
biomarker;
human xenobiotic metabolite;
plant metabolite
thiobarbituric acid
thiobarbituric acid: RN given refers to parent cpd. 2-thiobarbituric acid : A barbiturate, the structure of which is that of barbituric acid in which the oxygen at C-2 is replaced by sulfur.		6.9810barbituratesallergen;
reagent
selenium
Selenium: An element with the atomic symbol Se, atomic number 34, and atomic weight 78.97. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.		2.0510chalcogen;
nonmetal atom		micronutrient
ConditionIndicatedRelationship StrengthStudiesTrials
Innate Inflammatory Response
[description not available]		02.0510
Inflammation
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.		02.0510
Diabetes Mellitus
A heterogeneous group of disorders characterized by HYPERGLYCEMIA and GLUCOSE INTOLERANCE.		01.9910
Sensitivity and Specificity
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)		02.420