Page last updated: 2024-12-09

(S)-monastrol

Description Research Excerpts Clinical Trials Roles Classes Pathways Study Profile Bioassays Related Drugs Related Conditions Protein Interactions Research Growth Market Indicators

## (S)-Monastrol: A Powerful Tool for Cell Division Research

(S)-Monastrol is a **small-molecule inhibitor** that specifically targets **kinesin Eg5**, a key protein responsible for **microtubule-based movement** during **mitosis**, the process of cell division.

**Here's why it's important for research:**

* **Understanding Cell Division:** Monastrol blocks Eg5's function, preventing the proper separation of chromosomes during mitosis. This leads to **arrest of the cell cycle**, allowing researchers to study the **mechanisms of cell division** in detail.
* **Investigating Cancer:** Since uncontrolled cell division is a hallmark of cancer, monastrol is a valuable tool for **studying the role of Eg5 in tumor growth and development**. Researchers can use monastrol to investigate potential **anti-cancer targets** that disrupt the function of Eg5.
* **Developmental Biology Research:** Monastrol's ability to block mitosis makes it useful for **understanding embryonic development**. By inhibiting cell division in specific tissues, researchers can investigate the **role of Eg5 in cell fate determination and tissue morphogenesis**.
* **Probing Cellular Processes:** Monastrol is also used in **drug discovery and development** to identify new **therapeutic targets** for various diseases. Researchers can use monastrol to **screen for compounds that interact with Eg5** and potentially have therapeutic applications.

**Overall, (S)-monastrol is a powerful tool that has significantly advanced our understanding of cell division and its implications in various biological processes. It continues to play a crucial role in research, paving the way for new discoveries and therapeutic interventions.**

Cross-References

ID SourceID
PubMed CID794323
CHEBI ID75384
SCHEMBL ID74457

Synonyms (20)

Synonym
(4s)-monastrol
5-pyrimidinecarboxylic acid, 1,2,3,4-tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-, ethyl ester, (4s)- (9ci)
5-pyrimidinecarboxylic acid, 1,2,3,4-tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-, ethyl ester, (4s)-
(+)-monastrol
1Q0B
DB04331
chebi:75384 ,
s-menastrol
ethyl (4s)-4-(3-hydroxyphenyl)-6-methyl-2-sulfanylidene-1,2,3,4-tetrahydropyrimidine-5-carboxylate
ethyl (4s)-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate
SCHEMBL74457
unii-6bsm97yz8g
pilocereine ethyl ether [mi]
monastrol [mi]
monastrol, (+)-
6BSM97YZ8G ,
(s)-ethyl 1,2,3,4-tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxopyrimidine-5-carboxylate
ethyl (s)-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate
Q27463725
EN300-18755992
[information is derived through text-mining from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Drug Classes (1)

ClassDescription
ethyl 4-(3-hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carboxylateA member of the class of thioureas that is 3,4-dihydropyrimidine-2(1)-thione substituted by a 3-hydroxyphenyl group at position 4, an ethoxycarbonyl group at position 5, and a methyl group at position 6.
[compound class information is derived from Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

Protein Targets (2)

Inhibition Measurements

ProteinTaxonomyMeasurementAverageMin (ref.)Avg (ref.)Max (ref.)Bioassay(s)
Chain A, Kinesin-like protein KIF11Homo sapiens (human)IC50 (µMol)6.70006.70006.70006.7000AID977608
Kinesin-like protein KIF11Homo sapiens (human)IC50 (µMol)10.00000.00011.405710.0000AID242686; AID310787
[prepared from compound, protein, and bioassay information from National Library of Medicine (NLM), extracted Dec-2023]

Biological Processes (9)

Processvia Protein(s)Taxonomy
mitotic cell cycleKinesin-like protein KIF11Homo sapiens (human)
microtubule-based movementKinesin-like protein KIF11Homo sapiens (human)
spindle organizationKinesin-like protein KIF11Homo sapiens (human)
mitotic spindle organizationKinesin-like protein KIF11Homo sapiens (human)
mitotic centrosome separationKinesin-like protein KIF11Homo sapiens (human)
regulation of mitotic centrosome separationKinesin-like protein KIF11Homo sapiens (human)
cell divisionKinesin-like protein KIF11Homo sapiens (human)
mitotic spindle assemblyKinesin-like protein KIF11Homo sapiens (human)
spindle elongationKinesin-like protein KIF11Homo sapiens (human)
[Information is prepared from geneontology information from the June-17-2024 release]

Molecular Functions (6)

Processvia Protein(s)Taxonomy
microtubule motor activityKinesin-like protein KIF11Homo sapiens (human)
protein bindingKinesin-like protein KIF11Homo sapiens (human)
ATP bindingKinesin-like protein KIF11Homo sapiens (human)
microtubule bindingKinesin-like protein KIF11Homo sapiens (human)
protein kinase bindingKinesin-like protein KIF11Homo sapiens (human)
plus-end-directed microtubule motor activityKinesin-like protein KIF11Homo sapiens (human)
[Information is prepared from geneontology information from the June-17-2024 release]

Ceullar Components (10)

Processvia Protein(s)Taxonomy
spindle poleKinesin-like protein KIF11Homo sapiens (human)
spindle microtubuleKinesin-like protein KIF11Homo sapiens (human)
spindleKinesin-like protein KIF11Homo sapiens (human)
cytosolKinesin-like protein KIF11Homo sapiens (human)
microtubuleKinesin-like protein KIF11Homo sapiens (human)
membraneKinesin-like protein KIF11Homo sapiens (human)
mitotic spindleKinesin-like protein KIF11Homo sapiens (human)
kinesin complexKinesin-like protein KIF11Homo sapiens (human)
protein-containing complexKinesin-like protein KIF11Homo sapiens (human)
nucleusKinesin-like protein KIF11Homo sapiens (human)
mitotic spindleKinesin-like protein KIF11Homo sapiens (human)
[Information is prepared from geneontology information from the June-17-2024 release]

Bioassays (19)

Assay IDTitleYearJournalArticle
AID586659Upregulation of P-glycoprotein mRNA expression in pig LLC-PK1 cells2011Journal of medicinal chemistry, Mar-24, Volume: 54, Issue:6
Structure-activity relationship and multidrug resistance study of new S-trityl-L-cysteine derivatives as inhibitors of Eg5.
AID586595Ratio of GI50 for pig L-MDR1 cells overexpressing human P-glycoprotein to GI50 for pig LLC-PK1 cells2011Journal of medicinal chemistry, Mar-24, Volume: 54, Issue:6
Structure-activity relationship and multidrug resistance study of new S-trityl-L-cysteine derivatives as inhibitors of Eg5.
AID1377668Modulation of [3H]NCS-382 binding to alpha4betadelta GABAA receptor in rat cortical membranes after 1 hr by TopCount scintillation counting method2017European journal of medicinal chemistry, Sep-29, Volume: 138Monastrol, a 3,4-dihydropyrimidin-2(1H)-thione, as structural scaffold for the development of modulators for GHB high-affinity binding sites and α
AID654130Growth inhibition of human HT-29 cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID242686In vitro inhibitory concentration towards kinesin spindle protein activity of ATP hydrolysis in the presence of microtubules measured by ATPase assay (n=3)2005Bioorganic & medicinal chemistry letters, Apr-15, Volume: 15, Issue:8
Kinesin spindle protein (KSP) inhibitors. Part 1: The discovery of 3,5-diaryl-4,5-dihydropyrazoles as potent and selective inhibitors of the mitotic kinesin KSP.
AID654125Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID586660Upregulation of P-glycoprotein mRNA expression in mouse P388 cells2011Journal of medicinal chemistry, Mar-24, Volume: 54, Issue:6
Structure-activity relationship and multidrug resistance study of new S-trityl-L-cysteine derivatives as inhibitors of Eg5.
AID654124Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID586658Ratio of inhibition of growth in P-glycoprotein overexpressing mouse P388 cells to inhibition of growth in mouse P3882011Journal of medicinal chemistry, Mar-24, Volume: 54, Issue:6
Structure-activity relationship and multidrug resistance study of new S-trityl-L-cysteine derivatives as inhibitors of Eg5.
AID654127Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID1377669Modulation of [3H]NCS-382 binding to alpha4betadelta GABAA receptor in rat cortical membranes assessed as [3H]NCS-382 binding at 100 uM after 1 hr by TopCount scintillation counting method relative to control2017European journal of medicinal chemistry, Sep-29, Volume: 138Monastrol, a 3,4-dihydropyrimidin-2(1H)-thione, as structural scaffold for the development of modulators for GHB high-affinity binding sites and α
AID654128Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID654115Antioxidant activity assessed as scavenging of DPPH at 160 uM after 30 mins by spectrophotometry2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID654129Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID310787Inhibition of KSP2007Bioorganic & medicinal chemistry letters, Feb-01, Volume: 17, Issue:3
Pharmacophore identification of KSP inhibitors.
AID1153368Inhibition pig alpha-amylase at 50 to 300 ug/ml pretreated for 30 mins followed by addition of 1% starch for 10 mins by spectrometer2014Bioorganic & medicinal chemistry letters, Jul-01, Volume: 24, Issue:13
Graphite catalyzed solvent free synthesis of dihydropyrimidin-2(1H)-ones/thiones and their antidiabetic activity.
AID654126Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay2012Bioorganic & medicinal chemistry, Apr-15, Volume: 20, Issue:8
Free radical scavenging and antiproliferative properties of Biginelli adducts.
AID1811Experimentally measured binding affinity data derived from PDB2004Journal of molecular biology, Jan-09, Volume: 335, Issue:2
Inhibition of a mitotic motor protein: where, how, and conformational consequences.
AID977608Experimentally measured binding affinity data (IC50) for protein-ligand complexes derived from PDB2004Journal of molecular biology, Jan-09, Volume: 335, Issue:2
Inhibition of a mitotic motor protein: where, how, and conformational consequences.
[information is prepared from bioassay data collected from National Library of Medicine (NLM), extracted Dec-2023]

Research

Studies (7)

TimeframeStudies, This Drug (%)All Drugs %
pre-19900 (0.00)18.7374
1990's0 (0.00)18.2507
2000's3 (42.86)29.6817
2010's4 (57.14)24.3611
2020's0 (0.00)2.80
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Market Indicators

Research Demand Index: 12.51

According to the monthly volume, diversity, and competition of internet searches for this compound, as well the volume and growth of publications, there is estimated to be weak demand-to-supply ratio for research on this compound.

MetricThis Compound (vs All)
Research Demand Index12.51 (24.57)
Research Supply Index2.08 (2.92)
Research Growth Index4.59 (4.65)
Search Engine Demand Index0.00 (26.88)
Search Engine Supply Index0.00 (0.95)

This Compound (12.51)

All Compounds (24.57)

Study Types

Publication TypeThis drug (%)All Drugs (%)
Trials0 (0.00%)5.53%
Reviews0 (0.00%)6.00%
Case Studies0 (0.00%)4.05%
Observational0 (0.00%)0.25%
Other7 (100.00%)84.16%
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]